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Mesoscopic epifluorescence tomography: Reconstruction of superficial and deep fluorescence in highly-scattering media.
Opt. Express. 18, 8422-8429 (2010)
Mesoscopic Epifluorescence Tomography (MEFT) is a technique derived from Laminar Optical Tomography (LOT), determining fluorescence biodistribution by tomographic means in reflectance geometry. A pencil beam is scanned over the region of interest to excite fluorophores hidden within the tissue, while a CCD camera acquires images of reflected fluorescence emissions. This configuration is advantageous whenever transillumination of the specimen is not feasible, e. g., in the presence of skin chambers or when using wavelengths in the visible range where absorption is high. We present simulation and phantom studies recovering deep GFP-like fluorescence in highly scattering and strongly absorbing media with a penetration depth up to 10mm.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
LAMINAR OPTICAL TOMOGRAPHY; MOLECULAR TOMOGRAPHY; TURBID MEDIA; TRANSPORT; LIGHT
ISSN (print) / ISBN
1094-4087
e-ISSN
1094-4087
Zeitschrift
Optics Express
Quellenangaben
Band: 18,
Heft: 8,
Seiten: 8422-8429
Verlag
Optical Society of America (OSA)
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed