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Suchý, T.* ; Zieschang, C.* ; Popkova, Y.* ; Kaczmarek, I.* ; Weiner, J.* ; Liebing, A.D.* ; Çakir, M.V.* ; Landgraf, K.* ; Gericke, M.* ; Pospisilik, J.A.* ; Körner, A.* ; Heiker, J.T. ; Dannenberger, D.* ; Schiller, J.* ; Schöneberg, T.* ; Liebscher, I.* ; Thor, D.*

The repertoire of Adhesion G protein-coupled receptors in adipocytes and their functional relevance.

Int. J. Obes. 44, 2124–2136 (2020)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Background G protein-coupled receptors (GPCR) are well-characterized regulators of a plethora of physiological functions among them the modulation of adipogenesis and adipocyte function. The class of Adhesion GPCR (aGPCR) and their role in adipose tissue, however, is poorly studied. With respect to the demand for novel targets in obesity treatment, we present a comprehensive study on the expression and function of this enigmatic GPCR class during adipogenesis and in mature adipocytes. Methods The expression of all aGPCR representatives was determined by reanalyzing RNA-Seq data and by performing qPCR in different mouse and human adipose tissues under low- and high-fat conditions. The impact of aGPCR expression on adipocyte differentiation and lipid accumulation was studied by siRNA-mediated knockdown of all expressed members of this receptor class. The biological characteristics and function of mature adipocytes lacking selected aGPCR were analyzed by mass spectrometry and biochemical methods (lipolysis, glucose uptake, adiponectin secretion). Results More than ten aGPCR are significantly expressed in visceral and subcutaneous adipose tissues and several aGPCR are differentially regulated under high-caloric conditions in human and mouse. Receptor knockdown of six receptors resulted in an impaired adipogenesis indicating their expression is essential for proper adipogenesis. The altered lipid composition was studied in more detail for two representatives, ADGRG2/GPR64 and ADGRG6/GPR126. While GPR126 is mainly involved in adipocyte differentiation, GPR64 has an additional role in mature adipocytes by regulating metabolic processes. Conclusions Adhesion GPCR are significantly involved in qualitative and quantitative adipocyte lipid accumulation and can control lipolysis. Factors driving adipocyte formation and function are governed by signaling pathways induced by aGPCR yielding these receptors potential targets for treating obesity.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Subcutaneous Adipose-tissue; Chondroitin Sulfate; Insulin-resistance; Expression; Mechanisms; Adipogenesis; Angiogenesis; Adiponectin; Cells; Cd97
ISSN (print) / ISBN 0307-0565
e-ISSN 1476-5497
Zeitschrift International Journal of Obesity
Quellenangaben Band: 44, Heft: 10, Seiten: 2124–2136 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort Macmillan Building, 4 Crinan St, London N1 9xw, England
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Institute for Metabolism, Obesity and Vascular Research (HI-MAG)