PuSH - Publikationsserver des Helmholtz Zentrums München: Murine Gammaherpesvirus 68 Contains Two Functional Lytic Origins of Replication.

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Adler, H. ; Steer, B. ; Freimüller, K. ; Haas, J.*

Murine Gammaherpesvirus 68 Contains Two Functional Lytic Origins of Replication.

J. Virol. 81, 7300-7305 (2007)

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A 1.25-kbp DNA fragment from the right side of the genome containing the lytic origin of replication (oriLyt) of murine gammaherpesvirus 68 (MHV-68) has been identified by a plasmid replication assay. Here we show that a mutant MHV-68 with a deletion of an essential part of this oriLyt, generated by using an MHV-68 bacterial artificial chromosome, was only slightly attenuated and still able to replicate but that a mutant containing an additional deletion on the left side of the genome was replication deficient. The newly identified region was sufficient to support plasmid replication, thus providing evidence for a second oriLyt.

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