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Tietjen, J.R.* ; Zhang, D.W.* ; Rodriguez-Molina, J.B.* ; White, B.E.* ; Akhtar, M.S.* ; Heidemann, M. ; Li, X.* ; Chapman, R.D. ; Shokat, K.* ; Keles, S.* ; Eick, D. ; Ansari, A.Z.*

Chemical-genomic dissection of the CTD code.

Nat. Struct. Mol. Biol. 17, 1154-1161 (2010)
DOI PMC
Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Sequential modifications of the RNA polymerase II (Pol II) C-terminal domain (CTD) coordinate the stage-specific association and release of cellular machines during transcription. Here we examine the genome-wide distributions of the 'early' (phospho-Ser5 (Ser5-P)), 'mid' (Ser7-P) and 'late' (Ser2-P) CTD marks. We identify gene class-specific patterns and find widespread co-occurrence of the CTD marks. Contrary to its role in 3'-processing of noncoding RNA, the Ser7-P marks are placed early and retained until transcription termination at all Pol II-dependent genes. Chemical-genomic analysis reveals that the promoter-distal Ser7-P marks are not remnants of early phosphorylation but are placed anew by the CTD kinase Bur1. Consistent with the ability of Bur1 to facilitate transcription elongation and suppress cryptic transcription, high levels of Ser7-P are observed at highly transcribed genes. We propose that Ser7-P could facilitate elongation and suppress cryptic transcription.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter RNA-polymerase-II; Cryptic unstable transcripts; C-terminal domain; P-TEFB; Saccaromyces-cerevisiae; Bidirectional promoters; Histone modification; Basal transcription; Processing factors; Eukaryotic genome
Sprache englisch
Veröffentlichungsjahr 2010
HGF-Berichtsjahr 2010
ISSN (print) / ISBN 1545-9993
e-ISSN 1545-9985
Zeitschrift Nature Structural & Molecular Biology
Quellenangaben Band: 17, Heft: 9, Seiten: 1154-1161 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort New York, NY
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-501490-001
PubMed ID 20802488
DOI 10.1038/nsmb.1900
Scopus ID 77956344274
Erfassungsdatum 2010-10-07
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