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Kochanova, N.Y.* ; Schauer, T.* ; Mathias, G.P.* ; Lukacs, A.* ; Schmidt, A.* ; Flatley, A. ; Schepers, A. ; Thomae, A.W.* ; Imhof, A.*

A multi-layered structure of the interphase chromocenter revealed by proximity-based biotinylation.

Nucleic Acids Res. 48, 4161-4178 (2020)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
During interphase centromeres often coalesce into a small number of chromocenters, which can be visualized as distinct, DAPI dense nuclear domains. Intact chromocenters play a major role in maintaining genome stability as they stabilize the transcriptionally silent state of repetitive DNA while ensuring centromere function. Despite its biological importance, relatively little is known about the molecular composition of the chromocenter or the processes that mediate chromocenter formation and maintenance. To provide a deeper molecular insight into the composition of the chromocenter and to demonstrate the usefulness of proximity-based biotinylation as a tool to investigate those questions, we performed super resolution microscopy and proximity-based biotinylation experiments of three distinct proteins associated with the chromocenter in Drosophila. Our work revealed an intricate internal architecture of the chromocenter suggesting a complex multilayered structure of this intranuclear domain.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Drosophila-melanogaster; Heterochromatin Protein-1; Phase-separation; Satellite Dna; In-vivo; Chromatin; Mouse; Identification; Transcription; Localization
ISSN (print) / ISBN 0305-1048
e-ISSN 1362-4962
Zeitschrift Nucleic Acids Research
Quellenangaben Band: 48, Heft: 8, Seiten: 4161-4178 Artikelnummer: , Supplement: ,
Verlag Oxford University Press
Verlagsort Great Clarendon St, Oxford Ox2 6dp, England
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CF Monoclonal Antibodies (CF-MAB)
Förderungen DFG
Graduate School of Quantitative Biosciences Munich (QBM)
Deutsche Forschungsgemeinschaft (DFG)