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Ziegler, C.G.K.* ; Allon, S.J.* ; Nyquist, S.K.* ; Mbano, I.M.* ; Miao, V.N.* ; Tzouanas, C.N.* ; Cao, Y.* ; Yousif, A.S.* ; Bals, J.* ; Hauser, B.M.* ; Feldman, J.* ; Muus, C.* ; Wadsworth, M.H.* ; Kazer, S.W.* ; Hughes, T.K.* ; Doran, B.* ; Gatter, G.J.* ; Vukovic, M.* ; Taliaferro, F.* ; Mead, B.E.* ; Guo, Z.* ; Wang, J.P.* ; Gras, D.* ; Plaisant, M.* ; Ansari, M. ; Angelidis, I. ; Adler, H. ; Sucre, J.M.S.* ; Taylor, C.J.* ; Lin, B.* ; Waghray, A.* ; Mitsialis, V.* ; Dwyer, D.F.* ; Buchheit, K.M.* ; Boyce, J.A.* ; Barrett, N.A.* ; Laidlaw, T.M.* ; Carroll, S.L.* ; Colonna, L.* ; Tkachev, V.* ; Peterson, C.W.* ; Yu, A.* ; Zheng, H.B.* ; Gideon, H.P.* ; Winchell, C.G.* ; Lin, P.L.* ; Bingle, C.D.* ; Snapper, S.B.* ; Kropski, J.A.* ; Theis, F.J. ; Schiller, H. B. ; Zaragosi, L.E.* ; Barbry, P.* ; Leslie, A.* ; Kiem, H.P.* ; Flynn, J.L.* ; Fortune, S.M.* ; Berger, B.* ; Finberg, R.W.* ; Kean, L.S.* ; Garber, M.* ; Schmidt, A.G.* ; Lingwood, D.* ; Shalek, A.K.* ; Ordovas-Montanes, J.*

SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues.

Cell 181, 1016-1035 (2020)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
There is pressing urgency to understand the pathogenesis of the severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2), which causes the disease COVID-19. SARS-CoV-2 spike (S) protein binds angiotensin-converting enzyme 2 (ACE2), and in concert with host proteases, principally transmembrane serine protease 2 (TMPRSS2), promotes cellular entry. The cell subsets targeted by SARS-CoV-2 in host tissues and the factors that regulate ACE2 expression remain unknown. Here, we leverage human, non-human primate, and mouse single-cell RNA-sequencing (scRNA-seq) datasets across health and disease to uncover putative targets of SARS-CoV-2 among tissue-resident cell subsets. We identify ACE2 and TMPRSS2 co-expressing cells within lung type II pneumocytes, ileal absorptive enterocytes, and nasal goblet secretory cells. Strikingly, we discovered that ACE2 is a human interferon-stimulated gene (ISG) in vitro using airway epithelial cells and extend our findings to in vivo viral infections. Our data suggest that SARS-CoV-2 could exploit species-specific interferon-driven upregulation of ACE2, a tissue-protective mediator during lung injury, to enhance infection.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Ace2 ; Covid-19 ; Isg ; Sars-cov-2 ; Human ; Influenza ; Interferon ; Mouse ; Non-human Primate ; Scrna-seq; Angiotensin-converting Enzyme-2; Sars Coronavirus; I Interferons; Functional Receptor; Expression; Infection; Virus; Disease; Proteins; Replication
ISSN (print) / ISBN 0092-8674
e-ISSN 1097-4172
Zeitschrift Cell
Quellenangaben Band: 181, Heft: 5, Seiten: 1016-1035 Artikelnummer: , Supplement: ,
Verlag Cell Press
Verlagsort Cambridge, Mass.
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Lung Health and Immunity (LHI)
Research Unit Lung Repair and Regeneration (LRR)
Institute of Computational Biology (ICB)