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Grosch, J. ; Hilger, C.* ; Bilò, M.B.* ; Kler, S.* ; Schiener, M. ; Dittmar, G.* ; Bernardin, F.* ; Lesur, A.* ; Ollert, M.* ; Schmidt-Weber, C.B. ; Blank, S.

Shedding light on the venom proteomes of the allergy-relevant Hymenoptera Polistes dominula (European paper wasp) and Vespula spp. (yellow jacket).

Toxins 12:323 (2020)
Verlagsversion Forschungsdaten DOI
Open Access Gold
Creative Commons Lizenzvertrag
Allergic reactions to stings of Hymenoptera species can have serious or even fatal consequences. If the identification of the culprit insect is possible, venom-specific immunotherapy effectively cures Hymenoptera venom allergies. Although component-resolved diagnostics has strongly evolved in recent years, the differentiation between allergies to closely related species such asPolistes dominulaandVespulaspp. is still challenging. In order to generate the basis for new diagnostic and therapeutic strategies, this study aims at resolving the venom proteomes (venomes) of these species. The venoms ofP. dominulaandVespulaspp. (V. germanica,V. vulgaris) were analyzed by liquid chromatography-mass spectrometry. Resulting proteins were characterized regarding their function, localization and biochemical properties. The analyses yielded 157 proteins inVespulaspp. and 100 inP. dominulavenom; 48 proteins, including annotated allergens, were found in both samples. In addition to a variety of venom trace molecules, new allergen candidates such as icarapin-like protein and phospholipase A2 were identified. This study elucidates the venomes of closely related allergy-eliciting Hymenoptera species. The data indicates that relying on marker allergens to differentiate betweenP. dominulaandVespulaspp. venom allergy is probably insufficient and that strategies using cross-reactive major allergens could be more promising.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Allergen ; Hymenoptera Venom Allergy ; European Paper Wasp ; Polistes Dominulavenom ; Venom Proteome ; Vespulaspp ; Venom ; Yellow Jacket; Component-resolved Diagnosis; Dipeptidyl Peptidase Iv; Polistes-dominulus; Hymenoptera Venom; Molecular-cloning; Crystal-structure; Major Allergens; Mastoparan-b; Antigen 5; Helothermine
e-ISSN 2072-6651
Zeitschrift Toxins
Quellenangaben Band: 12, Heft: 5, Seiten: , Artikelnummer: 323 Supplement: ,
Verlag MDPI
Verlagsort Basel
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Allergy Research (IAF)