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Moya, N. ; Shahryari, A. ; Burtscher, I. ; Beckenbauer, J. ; Bakhti, M. ; Lickert, H.

Generation of a homozygous ARX nuclear CFP (ARX(nCFP/nCFP)) reporter human iPSC line (HMGUi001-A-4).

Stem Cell Res. 46:101874 (2020)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing alpha-cell differentiation. Here, we generate an ARX reporter iPSC line by 3' fusion of an intervening viral T2A sequence followed by a nuclear-localized histone 2B-cyan fluorescent protein (nCFP). The resulting cells have a normal karyotype and preserved pluripotency. In vitro differentiation of the ARX(nCFP/nCFP) reporter iPSCs towards the endocrine lineage confirmed the specific co-expression of the reporter protein in human glucagon' alpha-like cells. Thus, ARX(nCFP/nCFP) iPSC line will provide a powerful tool to monitor human a-cell progenitor differentiation as well as ARX(+) alpha-like cell function in vitro.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 1873-5061
e-ISSN 1876-7753
Zeitschrift Stem Cell Research
Quellenangaben Band: 46, Heft: , Seiten: , Artikelnummer: 101874 Supplement: ,
Verlag Elsevier
Verlagsort Radarweg 29, 1043 Nx Amsterdam, Netherlands
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Regeneration Research (IDR)
Institute of Stem Cell Research (ISF)