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Qadir, M.M.F.* ; Álvarez-Cubela, S.* ; Weitz, J.* ; Panzer, J.K.* ; Klein, D.* ; Moreno-Hernández, Y.* ; Cechin, S.* ; Tamayo, A.* ; Almaça, J.* ; Hiller, H.* ; Beery, M.* ; Kusmartseva, I.* ; Atkinson, M.* ; Speier, S. ; Ricordi, C.* ; Pugliese, A.* ; Caicedo, A.* ; Fraker, C.A.* ; Pastori, R.L.* ; Domínguez-Bendala, J.*

Long-term culture of human pancreatic slices as a model to study real-time islet regeneration.

Nat. Commun. 11:3265 (2020)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The culture of live pancreatic tissue slices is a powerful tool for the interrogation of physiology and pathology in an in vitro setting that retains near-intact cytoarchitecture. However, current culture conditions for human pancreatic slices (HPSs) have only been tested for short-term applications, which are not permissive for the long-term, longitudinal study of pancreatic endocrine regeneration. Using a culture system designed to mimic the physiological oxygenation of the pancreas, we demonstrate high viability and preserved endocrine and exocrine function in HPS for at least 10 days after sectioning. This extended lifespan allowed us to dynamically lineage trace and quantify the formation of insulin-producing cells in HPS from both non-diabetic and type 2 diabetic donors. This technology is expected to be of great impact for the conduct of real-time regeneration/developmental studies in the human pancreas. The ability to culture live pancreatic tissue slices for long periods of time would enable longitudinal studies ex vivo. Here the authors culture human and mouse pancreatic slices in a perfluorocarbon-based culture system and show stable endocrine and exocrine function for up to ten days in culture.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Beta-cell Differentiation; In-vitro; Acinar; Tissue; Viability; Situ
Sprache englisch
Veröffentlichungsjahr 2020
HGF-Berichtsjahr 2020
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Zeitschrift Nature Communications
Quellenangaben Band: 11, Heft: 1, Seiten: , Artikelnummer: 3265 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pancreatic Islet Research (IPI)
POF Topic(s) 90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502600-005
DOI 10.1038/s41467-020-17040-8
WOS ID WOS:000546312300003
Scopus ID 85087020112
PubMed ID 32601271
Erfassungsdatum 2020-07-16
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