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Wright, A.K.A.* ; Rao, S.* ; Range, S.* ; Eder, C.M.S. ; Hofer, T.P. ; Frankenberger, M. ; Kobzik, L.* ; Brightling, C.* ; Grigg, J.* ; Ziegler-Heitbrock, L.

Pivotal Advance: Expansion of small sputum macrophages in CF: Failure to express MARCO and mannose receptors.

J. Leukoc. Biol. 86, 479-489 (2009)
Verlagsversion DOI PMC
Free by publisher
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Macrophages in the airways form an important element of immune defense and inflammation. We analyzed induced sputum from airways of patients with CF for the types of macrophages present, their receptor expression, and phagocytic function. In samples from patients and age-matched controls, macrophages were analyzed by multicolor flow cytometry, scavenger receptor expression was studied at the protein and mRNA level, and receptor function was investigated using fluorescent particles. In adult patients with CF, we discovered a pronounced expansion of the small CD14+ DR+ CD68weak+ macrophages to 73 +/- 18% compared with 16 +/- 8% in healthy controls. Expression of the MARCO and CD206 (mannose receptor) was strongly reduced at the mRNA and protein level in sputum macrophages. Antibody-blocking studies showed that MARCO mediates phagocytosis of unopsonized particles. In line with reduced MARCO expression, sputum macrophages in CF showed a deficient uptake of particles (23 +/- 9% of cells) compared with healthy controls (71 +/- 15%). The deficiency of MARCO expression in the predominant small sputum macrophages in CF may lead to impaired clearance of inhaled particles with increased inflammation and damage to the CF lung.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter cystic fibrosis; phagocytosis; scavenger receptor; obstructive pulmonary-disease; cystic-fibrosis patients; alveolar macrophages; scavenger receptor; lung-disease; interferon-gamma; host-defense; inflammation; monocyte; mice
ISSN (print) / ISBN 0741-5400
e-ISSN 1938-3673
Zeitschrift Journal of Leukocyte Biology
Quellenangaben Band: 86, Heft: 3, Seiten: 479-489 Artikelnummer: , Supplement: ,
Verlag FASEB
Verlagsort BETHESDA
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Lung Health and Immunity (LHI)