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Karlina, R. ; Lutter, D. ; Miok, V. ; Fischer, D.S. ; Altun, I. ; Schöttl, T. ; Schorpp, K.K. ; Israel, A. ; Cero, C.* ; Johnson, J.W.* ; Kapser-Fischer, I. ; Böttcher, A. ; Keipert, S.* ; Feuchtinger, A. ; Graf, E. ; Strom, T.M. ; Walch, A.K. ; Lickert, H. ; Walzthoeni, T. ; Heinig, M. ; Theis, F.J. ; García-Cáceres, C. ; Cypess, A.M.* ; Ussar, S.

Identification and characterization of distinct brown adipocyte subtypes in C57BL/6J mice.

Life Sci. All. 4:e202000924 (2021)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Brown adipose tissue (BAT) plays an important role in the regulation of body weight and glucose homeostasis. Although increasing evidence supports white adipose tissue heterogeneity, little is known about heterogeneity within murine BAT. Recently, UCP1 high and low expressing brown adipocytes were identified, but a developmental origin of these subtypes has not been studied. To obtain more insights into brown preadipocyte heterogeneity, we use single-cell RNA sequencing of the BAT stromal vascular fraction of C57/BL6 mice and characterize brown preadipocyte and adipocyte clonal cell lines. Statistical analysis of gene expression profiles from brown preadipocyte and adipocyte clones identify markers distinguishing brown adipocyte subtypes. We confirm the presence of distinct brown adipocyte populations in vivo using the markers EIF5, TCF25, and BIN1. We also demonstrate that loss of Bin1 enhances UCP1 expression and mitochondrial respiration, suggesting that BIN1 marks dormant brown adipocytes. The existence of multiple brown adipocyte subtypes suggests distinct functional properties of BAT depending on its cellular composition, with potentially distinct functions in thermogenesis and the regulation of whole body energy homeostasis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Diet-induced Thermogenesis; Adipose-tissue; Transcriptional Control; Uncoupling Protein; Fat Development; Beige; White; Obesity; Adipogenesis; Initiation
ISSN (print) / ISBN 2575-1077
e-ISSN 2575-1077
Zeitschrift Life Science Alliance
Quellenangaben Band: 4, Heft: 1, Seiten: , Artikelnummer: e202000924 Supplement: ,
Verlag EMBO Press
Verlagsort Heidelberg
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Obesity (IDO)
Institute of Computational Biology (ICB)
Institute of Molecular Toxicology and Pharmacology (TOXI)
Institute of Diabetes and Regeneration Research (IDR)
Research Unit Analytical Pathology (AAP)
Institute of Human Genetics (IHG)
Förderungen European Research Council ERC Starting Grant (AstroNeuroCrosstalk)
project Aging and Metabolic Programming (AMPro)