PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Karlina, R. ; Lutter, D. ; Miok, V. ; Fischer, D.S. ; Altun, I. ; Schöttl, T. ; Schorpp, K.K. ; Israel, A. ; Cero, C.* ; Johnson, J.W.* ; Kapser-Fischer, I. ; Böttcher, A. ; Keipert, S.* ; Feuchtinger, A. ; Graf, E. ; Strom, T.M. ; Walch, A.K. ; Lickert, H. ; Walzthoeni, T. ; Heinig, M. ; Theis, F.J. ; García-Cáceres, C. ; Cypess, A.M.* ; Ussar, S.

Identification and characterization of distinct brown adipocyte subtypes in C57BL/6J mice.

Life Sci. All. 4:e202000924 (2021)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Brown adipose tissue (BAT) plays an important role in the regulation of body weight and glucose homeostasis. Although increasing evidence supports white adipose tissue heterogeneity, little is known about heterogeneity within murine BAT. Recently, UCP1 high and low expressing brown adipocytes were identified, but a developmental origin of these subtypes has not been studied. To obtain more insights into brown preadipocyte heterogeneity, we use single-cell RNA sequencing of the BAT stromal vascular fraction of C57/BL6 mice and characterize brown preadipocyte and adipocyte clonal cell lines. Statistical analysis of gene expression profiles from brown preadipocyte and adipocyte clones identify markers distinguishing brown adipocyte subtypes. We confirm the presence of distinct brown adipocyte populations in vivo using the markers EIF5, TCF25, and BIN1. We also demonstrate that loss of Bin1 enhances UCP1 expression and mitochondrial respiration, suggesting that BIN1 marks dormant brown adipocytes. The existence of multiple brown adipocyte subtypes suggests distinct functional properties of BAT depending on its cellular composition, with potentially distinct functions in thermogenesis and the regulation of whole body energy homeostasis.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
4.591
0.965
4
8
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Diet-induced Thermogenesis; Adipose-tissue; Transcriptional Control; Uncoupling Protein; Fat Development; Beige; White; Obesity; Adipogenesis; Initiation
Sprache englisch
Veröffentlichungsjahr 2021
Prepublished im Jahr 2020
HGF-Berichtsjahr 2020
ISSN (print) / ISBN 2575-1077
e-ISSN 2575-1077
Zeitschrift Life Science Alliance
Quellenangaben Band: 4, Heft: 1, Seiten: , Artikelnummer: e202000924 Supplement: ,
Verlag EMBO Press
Verlagsort Heidelberg
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Obesity (IDO)
Institute of Computational Biology (ICB)
Institute of Molecular Toxicology and Pharmacology (TOX)
Institute of Diabetes and Regeneration Research (IDR)
CF Pathology & Tissue Analytics (CF-PTA)
Institute of Human Genetics (IHG)
Research Unit Analytical Pathology (AAP)
POF Topic(s) 30201 - Metabolic Health
30205 - Bioengineering and Digital Health
30203 - Molecular Targets and Therapies
30505 - New Technologies for Biomedical Discoveries
30501 - Systemic Analysis of Genetic and Environmental Factors that Impact Health
90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
Enabling and Novel Technologies
Genetics and Epidemiology
PSP-Element(e) G-502296-001
G-502297-001
G-502200-001
G-503800-001
G-505293-001
G-502300-001
A-630600-001
G-500700-001
G-500390-001
G-553500-001
G-501900-224
G-509000-023
Förderungen European Research Council ERC Starting Grant (AstroNeuroCrosstalk)
project Aging and Metabolic Programming (AMPro)
DOI 10.26508/lsa.202000924
WOS ID WOS:000614606300014
Scopus ID 85097035062
PubMed ID 33257475
Erfassungsdatum 2020-12-15
[➜Korrektur melden]