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De Santis, M. ; Alsafadi, H.N.* ; Tas, S.* ; Bölükbas, D.A.* ; Prithiviraj, S.* ; Da Silva, I.A.N.* ; Mittendorfer, M.* ; Ota, C. ; Stegmayr, J.* ; Daoud, F.* ; Königshoff, M. ; Swärd, K.* ; Wood, J.A.* ; Tassieri, M.* ; Bourgine, P.E.* ; Lindstedt, S.* ; Mohlin, S.* ; Wagner, D.E.

Extracellular-matrix-reinforced bioinks for 3D bioprinting human tissue.

Adv. Mater. 33:2005476 (2021)
Postprint DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Recent advances in 3D bioprinting allow for generating intricate structures with dimensions relevant for human tissue, but suitable bioinks for producing translationally relevant tissue with complex geometries remain unidentified. Here, a tissue-specific hybrid bioink is described, composed of a natural polymer, alginate, reinforced with extracellular matrix derived from decellularized tissue (rECM). rECM has rheological and gelation properties beneficial for 3D bioprinting while retaining biologically inductive properties supporting tissue maturation ex vivo and in vivo. These bioinks are shear thinning, resist cell sedimentation, improve viability of multiple cell types, and enhance mechanical stability in hydrogels derived from them. 3D printed constructs generated from rECM bioinks suppress the foreign body response, are pro-angiogenic and support recipient-derived de novo blood vessel formation across the entire graft thickness in a murine model of transplant immunosuppression. Their proof-of-principle for generating human tissue is demonstrated by 3D bioprinting human airways composed of regionally specified primary human airway epithelial progenitor and smooth muscle cells. Airway lumens remained patent with viable cells for one month in vitro with evidence of differentiation into mature epithelial cell types found in native human airways. rECM bioinks are a promising new approach for generating functional human tissue using 3D bioprinting.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter 3d Bioprinting ; Biofabrication ; Bioinks ; Extracellular Matrix ; Tissue Engineering; High-throughput; Acellular Human; Hydrogels; Lung; Model
ISSN (print) / ISBN 0935-9648
e-ISSN 1521-4095
Zeitschrift Advanced materials
Quellenangaben Band: 33, Heft: 3, Seiten: , Artikelnummer: 2005476 Supplement: ,
Verlag Wiley
Verlagsort Weinheim
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Lung Repair and Regeneration (LRR)
Förderungen H2020 European Research Counci
European Research Council
Knut och Alice Wallenbergs Stiftelse
Cancerfonden
Barncancerfonden
Deutsche Zentrum für Lungenforschung
Deutsches Forschungszentrum für Gesundheit und Umwelt, Helmholtz Zentrum München