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Stefanie Buchberger, A.M.* ; Nitiu, R.* ; Pinther, M.* ; Graf, S.* ; Skodacek, D.* ; Regn, S. ; Kreutzer, K.* ; Storck, K.*

Fibrin gel suspended autologous chondrocytes as cell-based material for long-term injection laryngoplasty.

Laryngoscope, DOI: 10.1002/lary.29300 (2020)
Postprint DOI
Open Access Green
Objectives/Hypothesis: Injection laryngoplasty of materials for unilateral vocal-fold paralysis has shown various results regarding the long-term stability of the injected material. We evaluated a fibrin-gel based cell suspension with autologous chondrocytes in-vitro and in-vivo as long-term-stable vocal-fold augmentation material in an animal model. Study Design: This study compises an in vitro cell-culture part as well as an in vivo animal study with New Zealand White Rabbits. Methods: In in-vitro experiments, auricular chondrocytes harvested from 24 New Zealand White Rabbits cadavers were cultivated in pellet cultures to evaluate cartilage formation for 4 weeks using long-term-stable fibrin gel as carrier. Injectability and injection volume for the laryngoplasty was determined in-vitro using harvested cadaveric larynxes. In-vivo 24 Rabbits were biopsied for elastic cartilage of the ear and autologous P1 cells were injected lateral of one vocal cord into the paraglottic space suspended in a long-term-stable fibrin gel. Histologic evaluation was performed after 2, 4, 12, and 24 weeks. Results: During 12-week pellet culture, we found extracellular matrix formation and weight-stable cartilage of mature appearance. In-vivo, mature cartilage was found in two larynxes (n = 6) at 4 weeks, in four (n = 6) at 12 weeks, and in five (n = 6) at 24 weeks mostly located in the paraglottic space and sometimes with spurs into the vocalis muscle. Surrounding tissue was often infiltrated with inflammatory cells. Material tended to dislocate through the cricothyroid space into the extraglottic surrounding tissue. Conclusions: A cell-based approach with chondrocytes for permanent vocal-fold augmentation has not previously been reported. We have achieved the formation of structurally mature cartilage in the paraglottic space, but this is accompanied by difficulties with dislocated material, deformation of the augmentation, and inflammation. Level of Evidence: N/A Laryngoscope, 2020.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Chondrocytes ; Injection Laryngoplasty ; Unilateral Vocal Cord Paralysis ; Vocal Cord Augmentation; Vocal Fold Augmentation; Laryngeal Nerve Paralysis; Cross-linked Hyaluronan; Foreign-body Reaction; Viscoelastic Properties; Intracordal Injection; Glottal Insufficiency; Cartilage Injection; Auricular Cartilage; Acid
ISSN (print) / ISBN 0023-852X
e-ISSN 1531-4995
Zeitschrift Laryngoscope, The
Verlag Lippincott Williams & Wilkins
Verlagsort 111 River St, Hoboken 07030-5774, Nj Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Cancer (IDC)
Förderungen ProjektDEAL