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Duffner, C. ; Holzapfel, S.* ; Wunderlich, A.* ; Einsiedl, F.* ; Schloter, M. ; Schulz, S.

Dechloromonas and close relatives prevail hydrogenotrophic denitrification in stimulated microcosms with oxic aquifer material.

FEMS Microbiol. Ecol. 97:fiab004 (2021)
Verlagsversion DOI PMC
Free by publisher
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Globally occurring nitrate pollution in groundwater is harming the environment and human health. In situ hydrogen addition to stimulate denitrification has been proposed as a remediation strategy. However, observed nitrite accumulation and incomplete denitrification are severe drawbacks that possibly stem from the specific microbial community composition. We set up a microcosm experiment comprising sediment and groundwater from a nitrate polluted oxic oligotrophic aquifer. After the microcosms were sparged with hydrogen gas, samples were taken regularly within 122 h for nitrate and nitrite measurements, community composition analysis via 16S rRNA gene amplicon sequencing, and gene and transcript quantification via qPCR of reductase genes essential for complete denitrification. The highest nitrate reduction rates and greatest increase in bacterial abundance coincided with the 15.3-fold increase in Rhodocyclaceae, specifically six ASVs that are closely related to the genus Dechloromonas. The denitrification reductase genes napA, nirS, and clade I nosZ also increased significantly over the observation period. We conclude that taxa of the genus Dechloromonas are the prevailing hydrogenotrophic denitrifiers in this nitrate polluted aquifer and the ability of hydrogenotrophic denitrification under the given conditions is species-specific.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Rhodocyclaceae ; Denitrification Genes ; Groundwater ; Hydrogen Oxidation ; Nitrate Pollution ; Remediation; Denitrifying Bacteria; Nitrate; Groundwater; Water; Perchlorate; Diversity; Patterns; Kinetics; Biofilm; Reveal
ISSN (print) / ISBN 0168-6496
e-ISSN 1574-6941
Zeitschrift FEMS Microbiology Ecology
Quellenangaben Band: 97, Heft: 3, Seiten: , Artikelnummer: fiab004 Supplement: ,
Verlag Wiley
Verlagsort Oxford
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Comparative Microbiome Analysis (COMI)
Förderungen TUM International Graduate School of Science and Engineering(IGSSE)