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Gengatharan, A.* ; Malvaut, S.* ; Marymonchyk, A.* ; Ghareghani, M.* ; Snapyan, M.* ; Fischer-Sternjak, J. ; Ninkovic, J. ; Götz, M. ; Saghatelyan, A.*

Adult neural stem cell activation in mice is regulated by the day/night cycle and intracellular calcium dynamics.

Cell 184, 709-722.e13 (2021)
Verlagsversion Postprint Forschungsdaten DOI PMC
Open Access Green
Neural stem cells (NSCs) in the adult brain transit from the quiescent state to proliferation to produce new neurons. The mechanisms regulating this transition in freely behaving animals are, however, poorly understood. We customized in vivo imaging protocols to follow NSCs for several days up to months, observing their activation kinetics in freely behaving mice. Strikingly, NSC division is more frequent during daylight and is inhibited by darkness-induced melatonin signaling. The inhibition of melatonin receptors affected intracellular Ca2+ dynamics and promoted NSC activation. We further discovered a Ca2+ signature of quiescent versus activated NSCs and showed that several microenvironmental signals converge on intracellular Ca2+ pathways to regulate NSC quiescence and activation. In vivo NSC-specific optogenetic modulation of Ca2+ fluxes to mimic quiescent-state-like Ca2+ dynamics in freely behaving mice blocked NSC activation and maintained their quiescence, pointing to the regulatory mechanisms mediating NSC activation in freely behaving animals.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Crispr-cas9 ; Ca(2+) Signaling ; G-proteins ; Nscs ; Circadian Rhythm ; In vivo Imaging ; Melatonin ; Mini-endoscopes ; Neural Stem Cells ; Subventricular Zone
ISSN (print) / ISBN 0092-8674
e-ISSN 1097-4172
Zeitschrift Cell
Quellenangaben Band: 184, Heft: 3, Seiten: 709-722.e13 Artikelnummer: , Supplement: ,
Verlag Cell Press
Verlagsort Cambridge, Mass.
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Stem Cell Research (ISF)
Förderungen Canadian Institute of Health Research (CIHR)
Roger de Spoelberch Foundation