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Isolation of proteins from extracellular vesicles (EVs) for mass spectrometry-based proteomic analyses.
In: Proteomic Profiling. Berlin [u.a.]: Springer, 2021. 207-212 (Methods Mol. Biol. ; 2261)
Extracellular vesicles (EVs) are freely circulating nano/micrometer-sized membrane-bound vesicles released by various cell types. Their cargo consists of proteins, lipids, metabolites, and different types of RNA molecules reflecting the origin of the secreting cell type or tissue. Since the EV cargo is constantly changing in response to pathological status or different environmental stressors, it has been extensively studied in the quest for biomarkers, especially in the cancer research. Mass spectrometry (MS)-based proteome analysis is a powerful tool to elucidate the protein cargo in EVs. This chapter describes and characterizes three MS-compatible lysis methods, namely by using urea, guanidium hydrochloride, and radioimmunoprecipitation buffer for isolating proteins from EVs.
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Publikationstyp
Artikel: Sammelbandbeitrag/Buchkapitel
Schlagwörter
Data-dependent Acquisition ; Exosomes ; Extracellular Vesicles (evs) ; Mass Spectrometry ; Proteomics ; Sample Preparation
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Bandtitel
Proteomic Profiling
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 2261,
Seiten: 207-212
Verlag
Springer
Verlagsort
Berlin [u.a.]
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Radiation Biology (ISB)