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Kovářová, M. ; Kalbacher, H.* ; Peter, A. ; Häring, H.-U. ; Didangelos, T.* ; Stefan, N. ; Birkenfeld, A.L. ; Schleicher, E. ; Kantartzis, K.

Detection and characterization of phosphorylation, glycosylation, and fatty acid bound to fetuin A in human blood.

J. Clin. Med. 10:411 (2021)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The hepatokine fetuin A (Fet A) has been associated with diverse pathological states such as insulin resistance, type 2 diabetes, macrovascular disease, and systemic ectopic and vascular calcification. Fet A may also play a role in tumor growth and metastasis. The biological activity of Fet A may be affected by various modifications, including phosphorylation, O- and N-glycosylation and fatty acid binding. We developed an antibody-based assay for the detection of Fet A phosphorylated at serine 312. Fatty acid pattern was determined by gas chromatography. Using the antibody, we found that the phosphorylation was stable in human plasma or serum at room temperature for 8 h. We observed that Fet A is present in several glycosylation forms in human plasma, but the extent of Ser312 phosphorylation was not associated with glycosylation. The phosphorylation pattern did not change during an oral glucose tolerance test (0-120 min). We further found that human Fet A binds preferentially saturated fatty acids (>90%) at the expense of mono- and poly-unsaturated fatty acids. Our results indicate that different molecular species of Fet A are present in human plasma and that these different modifications may determine the different biological effects of Fet A.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Fam20c ; Fatty Acid Bound To Fetuin A ; Fatty Liver ; Fetuin A ; Glycosylation ; Obesity ; Phosphorylation
ISSN (print) / ISBN 2077-0383
e-ISSN 2077-0383
Zeitschrift Journal of Clinical Medicine
Quellenangaben Band: 10, Heft: 3, Seiten: , Artikelnummer: 411 Supplement: ,
Verlag MDPI
Verlagsort Basel
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes Research and Metabolic Diseases (IDM)
Förderungen German Center for Diabetes Research (DZD e.V)
German Federal Ministry of Education and Research (BMBF)