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A method for the establishment and characterization of mouse lung adenocarcinoma cell lines that mimic traits of human adenocarcinomas.
In: Lung Cancer. Berlin [u.a.]: Springer, 2021. 175-186 (Methods Mol. Biol. ; 2279)
Lung adenocarcinoma (LADC) is the leading cause of cancer death worldwide and is largely inflicted by carcinogens contained in tobacco smoke. The generation of cell lines mimicking traits of human LADC will profoundly advance our understanding of the pathobiology of the disease, as they offer an easy and valuable tool to study the cellular and molecular aspects of carcinogenesis. Here we describe a detailed protocol for the generation of such cell lines, following the exposure of experimental mouse strains to different tobacco carcinogens and isolation of the resulting lung tumors.
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Publikationstyp
Artikel: Sammelbandbeitrag/Buchkapitel
Schlagwörter
Chemical Carcinogenesis ; Lung Adenocarcinoma ; Mouse Cell Lines ; Smoke-induced Carcinogenesis ; Tobacco Smoke
Sprache
englisch
Veröffentlichungsjahr
2021
HGF-Berichtsjahr
2021
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Bandtitel
Lung Cancer
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 2279,
Seiten: 175-186
Verlag
Springer
Verlagsort
Berlin [u.a.]
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Lung Health and Immunity (LHI)
POF Topic(s)
30202 - Environmental Health
Forschungsfeld(er)
Lung Research
PSP-Element(e)
G-501600-003
Scopus ID
85102496491
PubMed ID
33683694
Erfassungsdatum
2021-04-26