PuSH - Publikationsserver des Helmholtz Zentrums München: Multiplexed serum antibody screening platform using virus extracts from endemic <em>Coronaviridae</em> and SARS-CoV-2.

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Fink, S.* ; Ruoff, F.* ; Stahl, A.* ; Becker, M.* ; Kaiser, P.* ; Traenkle, B.* ; Junker, D.* ; Weise, F.* ; Ruetalo, N.* ; Hörber, S. ; Peter, A. ; Nelde, A.* ; Walz, J.* ; Krause, G.* ; Baillot, A.* ; Schenke-Layland, K.* ; Joos, T.O.* ; Rothbauer, U.* ; Schneiderhan-Marra, N.* ; Schindler, M.* ; Templin, M.F.*

Multiplexed serum antibody screening platform using virus extracts from endemic Coronaviridae and SARS-CoV-2.

ACS Infect. Dis. 7, 1596-1606 (2021)

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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.

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The presence of antibodies against endemic coronaviruses has been linked to disease severity after SARS-CoV-2 infection. Assays capable of concomitantly detecting antibodies against endemic coronaviridae such as OC43, 229E, NL63, and SARS-CoV-2 may help to elucidate this question. We developed a serum screening platform using a bead-based Western blot system called DigiWest, capable of running hundreds of assays using microgram amounts of protein prepared directly from different viruses. Characterization of the immunoassay for detection of SARS-CoV-2 specific antibodies revealed a sensitivity of 90.3% and a diagnostic specificity of 98.1%. Concordance analysis with the SARS-CoV-2 immunoassays available by Roche, Siemens, and Euroimmun indicates comparable assay performances (Cohen's κ ranging from 0.8874 to 0.9508). Analogous assays for OC43, 229E, and NL63 were established and combined into one multiplex with the SARS-CoV-2 assay. Seroreactivity for different coronaviruses was detected with high incidence, and the multiplex assay was adapted for serum screening.

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