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Muller, L.A.H.* ; Ballhausen, M.B.* ; Andrade Linares, D.R. ; Pinek, L.* ; Golubeva, P.* ; Rillig, M.C.*

Fungus-bacterium associations are widespread in fungal cultures isolated from a semi-arid natural grassland in Germany.

FEMS Microbiol. Ecol. 97:fiab059 (2021)
Postprint Forschungsdaten DOI PMC
Open Access Green
We report on a study that aimed at establishing a large soil-fungal culture collection spanning a wide taxonomic diversity and systematically screening the collection for bacterial associations. Fungal cultures were isolated from soil samples obtained from a natural grassland in eastern Germany and bacterial associations were assessed by PCR-amplification and sequencing of bacterial 16S rRNA. In addition, intraspecies genetic diversities of a subset of the isolated species were estimated by double-digest restriction associated DNA sequencing. A total of 688 fungal cultures, representing at least 106 fungal species from 36 different families, were obtained and even though clonal isolates were identified in almost all fungal species subjected to ddRAD-seq, relatively high genetic diversities could be observed in some of the isolated species. Sixty-nine % of the fungal isolates in our collection were found to be associated with bacteria and the most commonly identified bacterial genera were Pelomonas, Enterobacter and Burkholderia. Our results indicate that bacterial associations commonly occur in soil fungi, even if antibiotics are being applied during the isolation process, and provide a basis for the use of our culture collection in ecological experiments that want to acknowledge the importance of intraspecies genetic diversity.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Bacteria ; Fungal-bacterial Interaction ; Soil Fungi ; Ddrad Sequencing; Sp Nov.; Mycorrhizal; Identification; Diversity; Sequence; Hyphae
ISSN (print) / ISBN 0168-6496
e-ISSN 1574-6941
Zeitschrift FEMS Microbiology Ecology
Quellenangaben Band: 97, Heft: 5, Seiten: , Artikelnummer: fiab059 Supplement: ,
Verlag Wiley
Verlagsort Oxford
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Comparative Microbiome Analysis (COMI)
Förderungen European Research Council