PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Vara-Perez, M.* ; Rossi, M.* ; van den Haute, C.* ; Maes, H.* ; Sassano, M.L.* ; Venkataramani, V.* ; Michalke, B. ; Romano, E.* ; Rillaerts, K.* ; Garg, A.D.* ; Schepkens, C.* ; Bosisio, F.M.* ; Wouters, J.* ; Oliveira, A.I.* ; Vangheluwe, P.* ; Annaert, W.* ; Swinnen, J.* ; Colet, J.M.* ; van den Oord, J.J.* ; Fendt, S.* ; Mazzone, M.* ; Agostinis, P.*

BNIP3 promotes HIF-1 alpha-driven melanoma growth by curbing intracellular iron homeostasis.

EMBO J. 40:e106214 (2021)
Verlagsversion DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
BNIP3 is a mitophagy receptor with context-dependent roles in cancer, but whether and how it modulates melanoma growth in vivo remains unknown. Here, we found that elevated BNIP3 levels correlated with poorer melanoma patient's survival and depletion of BNIP3 in B16-F10 melanoma cells compromised tumor growth in vivo. BNIP3 depletion halted mitophagy and enforced a PHD2-mediated downregulation of HIF-1 alpha and its glycolytic program both in vitro and in vivo. Mechanistically, we found that BNIP3-deprived melanoma cells displayed increased intracellular iron levels caused by heightened NCOA4-mediated ferritinophagy, which fostered PHD2-mediated HIF-1 alpha destabilization. These effects were not phenocopied by ATG5 or NIX silencing. Restoring HIF-1 alpha levels in BNIP3-depleted melanoma cells rescued their metabolic phenotype and tumor growth in vivo, but did not affect NCOA4 turnover, underscoring that these BNIP3 effects are not secondary to HIF-1 alpha. These results unravel an unexpected role of BNIP3 as upstream regulator of the pro-tumorigenic HIF-1 alpha glycolytic program in melanoma cells.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
11.598
0.000
4
5
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Bnip3 ; Ferritinophagy ; Hif‐ ; 1α ; Melanoma ; Metabolism
Sprache englisch
Veröffentlichungsjahr 2021
HGF-Berichtsjahr 2021
ISSN (print) / ISBN 0261-4189
e-ISSN 1460-2075
Zeitschrift EMBO Journal, The
Quellenangaben Band: 40, Heft: 10, Seiten: , Artikelnummer: e106214 Supplement: ,
Verlag Wiley
Verlagsort Heidelberg, Germany
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit BioGeoChemistry and Analytics (BGC)
POF Topic(s) 30202 - Environmental Health
Forschungsfeld(er) Environmental Sciences
PSP-Element(e) G-504800-002
Förderungen Flemish Research Foundation (FWO-Vlaanderen)
EOS consortium
KU Leuven
Kom Op tegen Kanker (Stand up to Cancer)
Flemish cancer society
FWO
Stichting tegen Kanker
KU Leuven Methusalem
Stichting tegen Kanker (Foundation against Cancer)
Belgian Cancer Society
Deutsche Forschungsgemeinschaft (DFG) through the Priority Program "Ferroptosis: from Molecular Basics to Clinical Applications"
Hercules
FWO Doctoral Fellowship from the Flemish Research Foundation (FWO-Vlaanderen), Belgium
DOI 10.15252/embj.2020106214
WOS ID WOS:000645932700001
Scopus ID 85105173041
PubMed ID 33932034
Erfassungsdatum 2021-06-11
[➜Korrektur melden]