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Laaser, I. ; Theis, F.J. ; Hrabě de Angelis, M. ; Kolb, H.-J. ; Adamski, J.

Huge splicing frequency in human Y chromosomal UTY gene.

OMICS 15, 141-154 (2011)
Verlagsversion DOI PMC
Over 90% of human genes produce more than one mRNA by alternative splicing (AS). Human UTY (ubiquitously transcribed tetratricopeptide repeat protein on the chromosome Y) has six mRNA-transcripts. UTY is subject to interdisciplinary approaches such as Y chromosomal genetics or development of leukemia immunotherapy based on UTY-specific peptides. Investigating UTY expression in a normal and leukemic setting we discovered an exceptional splicing phenomenon fostering huge transcript diversity. Transcript sequencing identified 90 novel AS-events being almost randomly combined in 284 new transcripts. We uncovered a novel system of transcript architecture and genomic organization in UTY. On a basis of a new UTY-splicing multigraph including a mathematical model we calculated the theoretical yield to exceed 1.3 billion distinct transcripts. To our knowledge, this is the greatest estimated transcript diversity by AS. On protein level we demonstrated interaction of AS-derived proteins with new interactors by yeast-two-hybrid assay. For translational research we predicted new UTY-peptide candidates for leukemia therapy development. Our study provides new insights into the complexity of human alternative splicing and its potential contribution to the transcript diversity of the transcriptome.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter nonsense-mediated decay; male-specific region; messenger-rna decay; human transcriptome; proteins; generation; isoforms; cells
ISSN (print) / ISBN 1070-2830
e-ISSN 1557-8100
Zeitschrift OMICS
Quellenangaben Band: 15, Heft: 3, Seiten: 141-154 Artikelnummer: , Supplement: ,
Verlag Mary Ann Liebert
Verlagsort New Rochelle, USA
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Molekulare Endokrinologie und Metabolismus (MEM)
Institute of Experimental Genetics (IEG)
Institute of Bioinformatics and Systems Biology (IBIS)