PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Linder, A.* ; Bothe, V.* ; Linder, N.* ; Schwarzlmueller, P.* ; Dahlström, F.* ; Bartenhagen, C.* ; Dugas, M.* ; Pandey, D.* ; Thorn-Seshold, J. ; Boehmer, D.F.R.* ; Koenig, L.M.* ; Kobold, S.* ; Schnurr, M.* ; Raedler, J.* ; Spielmann, G.* ; Karimzadeh, H. ; Schmidt, A.* ; Endres, S. ; Rothenfußer, S.

Defective interfering genomes and the full-length viral genome trigger RIG-I after infection with vesicular stomatitis virus in a replication dependent manner.

Front. Immunol. 12:595390 (2021)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Replication competent vesicular stomatitis virus (VSV) is the basis of a vaccine against Ebola and VSV strains are developed as oncolytic viruses. Both functions depend on the ability of VSV to induce adequate amounts of interferon-α/β. It is therefore important to understand how VSV triggers interferon responses. VSV activates innate immunity via retinoic acid-inducible gene I (RIG-I), a sensor for viral RNA. Our results show that VSV needs to replicate for a robust interferon response. Analysis of RIG-I-associated RNA identified a copy-back defective-interfering (DI) genome and full-length viral genomes as main trigger of RIG-I. VSV stocks depleted of DI genomes lost most of their interferon-stimulating activity. The remaining full-length genome and leader-N-read-through sequences, however, still triggered RIG-I. Awareness for DI genomes as trigger of innate immune responses will help to standardize DI genome content and to purposefully deplete or use DI genomes as natural adjuvants in VSV-based therapeutics.
Impact Factor
Scopus SNIP
Altmetric
7.561
1.573
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Defective Interfering Genome ; Nucleic Acid Sensing ; Pathogen Associated Molecular Pattern (pamp) ; Pattern Recognition Receptor (prr) ; Retinoid Acid Inducible Gene I (rig-i) ; Vesicular Stomatitis Virus (vsv); Stranded-rna
Sprache englisch
Veröffentlichungsjahr 2021
HGF-Berichtsjahr 2021
ISSN (print) / ISBN 1664-3224
e-ISSN 1664-3224
Zeitschrift Frontiers in Immunology
Quellenangaben Band: 12, Heft: , Seiten: , Artikelnummer: 595390 Supplement: ,
Verlag Frontiers
Verlagsort Avenue Du Tribunal Federal 34, Lausanne, Ch-1015, Switzerland
Begutachtungsstatus Peer reviewed
Institut(e) Unit for Clinical Pharmacology (KKG-EKLiP)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-522100-001
Förderungen international doctoral program "iTarget: Immunotargeting of cancer" - Elite Network of Bavaria
Marie-Sklodowska-Curie Training Network for the Immunotherapy of Cancer (IMMUTRAIN) - H2020 program of the European Union
Else Kroner-Fresenius-Stiftung
Friedrich-Baur-Stiftung
Deutsche Forschungsgemeinschaft
DFG
Einheit fur Klinische Pharmakologie (EKLIP), Helmholtz Zentrum Munchen, Neuherberg, Germany
DOI 10.3389/fimmu.2021.595390
WOS ID WOS:000650031200001
Scopus ID 85105974726
PubMed ID 33995343
Erfassungsdatum 2021-06-28
[➜Korrektur melden]