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Sagmeister, S. ; Merl-Pham, J. ; Petrera, A. ; Deeg, C.A.* ; Hauck, S.M.

High glucose treatment promotes extracellular matrix proteome remodeling in Muller glial cells.

PeerJ 9:e11316 (2021)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Background. The underlying pathomechanisms in diabetic retinopathy (DR) remain incompletely understood. The aim of this study was to add to the current knowledge about the particular role of retinal Muller glial cells (RMG) in the initial processes of DR.Methods. Applying a quantitative proteomic workflow, we investigated changes of primary porcine RMG under short term high glucose treatment as well as glycolysis inhibition treatment.Results. We revealed significant changes in RMG proteome primarily in proteins building the extracellular matrix (ECM) indicating fundamental remodeling processes of ECM as novel rapid response to high glucose treatment. Among others, Osteopontin (SPP1) as well as its interacting integrins were significantly downregulated and organotypic retinal explant culture confirmed the selective loss of SPP1 in RMG upon treatment. Since SPP1 in the retina has been described neuroprotective for photoreceptors and functions against experimentally induced cell swelling, it's rapid loss under diabetic conditions may point to a direct involvement of RMG to the early neurodegenerative processes driving DR. Data are available via ProteomeXchange with identifier PXDO15879.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Diabetic Retinopathy ; Secreted Phosphoprotein 1 (spp1) ; Osteopontin ; Neurodegeneration ; Organotypic Explant Cultures ; Translational Large Animal Model ; Pig; Data-independent Acquisition; Diabetic-retinopathy; Identification; Proteins; Survival; Cultures; Retina; Edema; Model
ISSN (print) / ISBN 2167-8359
e-ISSN 2167-8359
Zeitschrift PeerJ
Quellenangaben Band: 9, Heft: , Seiten: , Artikelnummer: e11316 Supplement: ,
Verlag PeerJ
Verlagsort 341-345 Old St, Third Flr, London, Ec1v 9ll, England
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)
Förderungen Deutsche Forschungsgemeinschaft