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Hurtado, C.R.* ; Hurtado, G.R.* ; de Cena, G.L.* ; Queiroz, R.C.* ; Silva, A.V. ; Diniz, M.F.* ; Dos Santos, V.R.* ; Trava-Airoldi, V.* ; Baptista, M.d.S.* ; Tsolekile, N.* ; Oluwafemi, O.S.* ; Conceição, K.* ; Tada, D.B.*

Diamond nanoparticles-porphyrin mthpp conjugate as photosensitizing platform: Cytotoxicity and antibacterial activity.

Nanomaterials 11:1393 (2021)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Conjugation of photosensitizers (PS) with nanoparticles has been largely used as a strategy to stabilize PS in the biological medium resulting in photosensitizing nanoparticles of enhanced photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis (3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond nanoparticles (ND) by covalent bond. Nanoconjugate ND-mTHPP showed suitable stability in aqueous suspension with 58 nm of hydrodynamic diameter and Zeta potential of −23 mV. The antibacterial activity of ND-mTHPP was evaluated against Escherichia coli for different incubation times (0–24 h). The optimal activity was observed after 2 h of incubation and irradiation (660 nm; 51 J/cm ) performed right after the addition of ND-mTHPP (100 µg/mL) to the bacterial suspension. The inhibitory activity was 56% whereas ampicillin at the same conditions provided only 14% of bacterial growth inhibition. SEM images showed agglomerate of ND-mTHPP adsorbed on the bacterial cell wall, suggesting that the antimicrobial activity of ND-mTHPP was afforded by inducing membrane damage. Cytotoxicity against murine embryonic fibroblast cells (MEF) was also evaluated and ND-mTHPP was shown to be noncytotoxic since viability of cells cultured for 24 h in the presence of the nanoconjugate (100 µg/mL) was 78%. Considering the enhanced antibacterial activity and the absence of cytotoxic effect, it is possible to consider the ND-mTHPP nanoconjugate as promising platform for application in antimicrobial photodynamic therapy (aPDT). 2
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Drug-resistance ; Nanoconjugate ; Photodynamic Antimicrobial Therapy (apdt) ; Photosensitizers (ps); Photodynamic Therapy; Photophysicochemical Properties; Antimicrobial Activity; Surface Modification; Nanodiamond; Cancer; Functionalization; Phthalocyanine; Fluorescence; Metabolites
ISSN (print) / ISBN 2079-4991
e-ISSN 2079-4991
Zeitschrift Nanomaterials
Quellenangaben Band: 11, Heft: 6, Seiten: , Artikelnummer: 1393 Supplement: ,
Verlag MDPI
Verlagsort Basel
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Medicinal Chemistry (IMC)
Förderungen
Fundacao de Amparo ao Ensino e Pesquisa do Estado de Sao Paulo