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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Isolation and Eeectron microscopic analysis of liver cancer cell mitochondria.
In: Mitochondrial Medicine. Berlin [u.a.]: Springer, 2021. 277-287 (Methods Mol. Biol. ; 2277)
Isolation of mitochondria is a crucial method for examining molecular details of this organelle's manifold functions. Historically, mitochondrial isolations required large amounts of sample material which impeded their isolation from cultured cells. We have therefore developed a method allowing for controlled and reproducible isolation of intact and functional mitochondria from diverse cell types in culture. Here we provide a methodological update of this approach together with a protocol for the subsequent analysis of such isolated mitochondria by electron microscopy. Combining the isolation procedure with this powerful imaging method can reveal ultrastructural mitochondrial peculiarities in disease settings that might not be evident in intact cells and allows for assessment of mitochondrial membrane integrity and sample purity.
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Publikationstyp
Artikel: Sammelbandbeitrag/Buchkapitel
Schlagwörter
Balch Homogenizer ; Cell Culture ; Electron Microscopy ; Image Analysis ; Mitochondria
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Bandtitel
Mitochondrial Medicine
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 2277,
Seiten: 277-287
Verlag
Springer
Verlagsort
Berlin [u.a.]
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed