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Protocol for using heterologous spike-ins to normalize for technical variation in chromatin immunoprecipitation.
STAR Protoc. 2:100609 (2021)
Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous spike-ins from Drosophila chromatin as an internal control to the mice chromatin before immunoprecipitation to normalize for technical variation in ChIP-qPCR or ChIP-seq. The choice of spike-in depends on the evolutionary conservation of the protein of interest and the antibody used. For complete details on the use and execution of this protocol, please refer to Greulich et al. (2021).
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Cell Biology ; Chipseq ; Chromatin Immunoprecipitation (chip) ; Molecular Biology ; Sequence Analysis
e-ISSN
2666-1667
Zeitschrift
STAR Protocols
Quellenangaben
Band: 2,
Heft: 3,
Artikelnummer: 100609
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Diabetes and Cancer (IDC)