Multiparametric optimization of multispectral optoacoustic tomography for deep tissue imaging.
    
    
        
    
    
        
        Proc. SPIE 7564:75642Z (2010)
    
    
    
		
		
			
				Over the last decade fluorescent reporter technologies (both fluorescent probes and proteins) have become a very powerful imaging tool in everyday biomedical research. Multispectral optoacoustic tomography (MSOT) is an emerging imaging technology that can resolve fluorophore concentration in small animals situated in deep tissue by multispectral acquisition and processing of optoacoustic signals. In this work, we study the optimum operating conditions of MSOT in imaging fluorescence activity in small animals. The performance of various fluorochromes / fluorescent proteins is examined and it is shown that the new infrared fluorescent protein is an order of magnitude brighter than the red ones. Finally, wavelength reduction after principle component analysis shows, that accurate unmixing and 3D reconstruction of the distribution of fluorochromes is possible only with 2 or 3 wavelengths.
			
			
				
			
		 
		
			
				
					
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        Publikationstyp
        Artikel: Journalartikel
    
 
    
        Dokumenttyp
        Wissenschaftlicher Artikel
    
 
    
        Typ der Hochschulschrift
        
    
 
    
    
        Schlagwörter
        Fluorescent Protein ; Molecular Imaging ; Multispectral ; Photoacoustic ; Tomography
    
 
    
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        Sprache
        englisch
    
 
    
        Veröffentlichungsjahr
        2010
    
 
    
        Prepublished im Jahr 
        
    
 
    
        HGF-Berichtsjahr
        0
    
 
    
    
        ISSN (print) / ISBN
        0277-786X
    
 
    
        e-ISSN
        1996-756X
    
 
    
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	    Band: 7564,  
	    Heft: ,  
	    Seiten: ,  
	    Artikelnummer: 75642Z 
	    Supplement: ,  
	
    
 
  
        
            Reihe
            Proceedings of SPIE
        
 
        
            Verlag
            SPIE
        
 
        
            Verlagsort
            Bellingham, WA
        
 
	
        
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        Begutachtungsstatus
        Peer reviewed
    
 
     
    
        POF Topic(s)
        30205 - Bioengineering and Digital Health
    
 
    
        Forschungsfeld(er)
        Enabling and Novel Technologies
    
 
    
        PSP-Element(e)
        G-505500-001
    
 
    
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        Erfassungsdatum
        2011-08-30