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Tokarew, N.J.A.* ; Gosálvez, J.S.* ; Nottebrock, A.* ; Briukhovestka, D.* ; Endres, S. ; Cadilha, B.L.* ; Kobold, S.

Flow cytometry detection and quantification of CAR T cells into solid tumors.

Methods Cell Biol. 167, 99-122 (2022)
DOI PMC
Adoptive T cell therapy (ACT) is a therapeutic approach which employs genetically manipulated autologous T cells to target and eliminate a patient's malignancy. This novel therapeutic approach, when employing a chimeric antigen receptor (CAR) targeting CD19-expressing B cells, has shown remarkable success in treating acute B-cell lymphocytic leukemia. However, blood born malignancies represent only a fraction of cancers which affect patients. Unfortunately, the utilization of ACT to target solid malignancies has only shown marginal success rates. There are many known obstacles which hinder CAR T cell therapy in patients suffering from solid cancer, one notable obstacle is the effective trafficking of CAR T cells to the tumor site. With the rapid advancement of novel approaches and targets which may enhance CAR T cell infiltration into solid tumors, a standardized approach to assess and measure CAR T cell infiltration becomes imperative in order to compare these different approaches across platforms. Here we describe a flow cytometry method which enables the rapid detection and quantification of CAR T cells which have reached and entered the tumor mass following intravenous injection. Competence with single cell preparation and flow cytometry is required for optimal results.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Korrespondenzautor
Schlagwörter Adoptive T Cell Transfer ; Cancer Immunotherapy ; Car T Cells ; Flow Cytometry ; Immunotherapy ; Protocol ; T Cell Tracking
ISSN (print) / ISBN 0091-679X
Zeitschrift Methods in Cell Biology
Quellenangaben Band: 167, Heft: , Seiten: 99-122 Artikelnummer: , Supplement: ,
Verlag Elsevier
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Unit for Clinical Pharmacology (KKG-EKLiP)