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A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions.
STAR Protoc. 2:100700 (2021)
With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for inducing, testing, and studying the repair mechanisms associated with DNA double-strand breaks, interstrand cross-link repair, and single-strand break repair. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2017, 2020).
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Cancer ; Cell Biology ; Microscopy ; Molecular Biology
e-ISSN
2666-1667
Zeitschrift
STAR Protocols
Quellenangaben
Band: 2,
Heft: 3,
Artikelnummer: 100700
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Diabetes and Cancer (IDC)