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Aegilops tauschii genome assembly Aet v5.0 features greater sequence contiguity and improved annotation.
Genes Genomes Genetics G3 11:jkab325 (2021)
Aegilops tauschii is the donor of the D subgenome of hexaploid wheat and an important genetic resource. The reference-quality genome sequence Aet v4.0 for Ae. tauschii acc. AL8/78 was therefore an important milestone for wheat biology and breeding. Further advances in sequencing acc. AL8/78 and release of the Aet v5.0 sequence assembly are reported here. Two new optical maps were constructed and used in the revision of pseudomolecules. Gaps were closed with Pacific Biosciences long-read contigs, decreasing the gap number by 38,899. Transposable elements and protein-coding genes were reannotated. The number of annotated high-confidence genes was reduced from 39,635 in Aet v4.0 to 32,885 in Aet v5.0. A total of 2,245 biologically important genes, including those affecting plant phenology, grain quality, and tolerance of abiotic stresses in wheat was manually annotated and disease-resistance genes were annotated by a dedicated pipeline. Disease-resistance genes encoding nucleotide-binding site domains, receptor-like protein kinases, and receptor-like proteins were preferentially located in distal chromosome regions, whereas those encoding transmembrane coiled-coil proteins were dispersed more evenly along the chromosomes. Discovery, annotation, and expression analyses of microRNA (miRNA) precursors, mature miRNAs, and phasiRNAs are reported, including miRNA target genes. Other small RNAs, such as hc-siRNAs and tRFs, were characterized. These advances enhance the utility of the Ae. tauschii genome sequence for wheat genetics, biotechnology, and breeding.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Pacific Biosciences ; Disease Resistance ; Hc-sirna ; Mirna ; Optical Map ; Phasirna ; Trf ; Trna ; Transposable Elements; Polyploid Wheat; Small Rnas; Gene; Resistance; Diversity; Evolution; Diversification; Prediction; Ancient; Tool
ISSN (print) / ISBN
2160-1836
e-ISSN
2160-1836
Zeitschrift
Genes Genomes Genetics G3
Quellenangaben
Band: 11,
Heft: 12,
Artikelnummer: jkab325
Verlag
Genetics Society of America
Verlagsort
Pittsburgh, PA
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Förderungen
National Science Foundation