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Romano, M.* ; Bravin, A.* ; Mittone, A.* ; Eckhardt, A.* ; Barbone, G.E.* ; Sancey, L.* ; Dinkel, J.* ; Bartzsch, S. ; Ricke, J.* ; Alunni-Fabbroni, M.* ; Hirner-Eppeneder, H.* ; Karpov, D.* ; Giannini, C.* ; Bunk, O.* ; Bouchet, A.* ; Ruf, V.* ; Giese, A.* ; Coan, P.*

A multi-scale and multi-technique approach for the characterization of the effects of spatially fractionated X-ray radiation therapies in a preclinical model.

Cancers 13:4953 (2021)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The purpose of this study is to use a multi-technique approach to detect the effects of spatially fractionated X-ray Microbeam (MRT) and Minibeam Radiation Therapy (MB) and to compare them to seamless Broad Beam (BB) irradiation. Healthy-and Glioblastoma (GBM)-bearing male Fischer rats were irradiated in-vivo on the right brain hemisphere with MRT, MB and BB delivering three different doses for each irradiation geometry. Brains were analyzed post mortem by multi-scale X-ray Phase Contrast Imaging–Computed Tomography (XPCI-CT), histology, immunohistochemistry, X-ray Fluorescence (XRF), Small-and Wide-Angle X-ray Scattering (SAXS/WAXS). XPCI-CT discriminates with high sensitivity the effects of MRT, MB and BB irradiations on both healthy and GBM-bearing brains producing a first-time 3D visualization and morphological analysis of the radio-induced lesions, MRT and MB induced tissue ablations, the presence of hyperdense deposits within specific areas of the brain and tumor evolution or regression with respect to the evaluation made few days post-irradiation with an in-vivo magnetic resonance imaging session. Histology, immunohistochemistry, SAXS/WAXS and XRF allowed identification and classification of these deposits as hydroxyapatite crystals with the coexistence of Ca, P and Fe mineralization, and the multi-technique approach enabled the realization, for the first time, of the map of the differential radiosensitivity of the different brain areas treated with MRT and MB. 3D XPCI-CT datasets enabled also the quantification of tumor volumes and Ca/Fe deposits and their full-organ visualization. The multi-scale and multi-technique approach enabled a detailed visualization and classification in 3D of the radio-induced effects on brain tissues bringing new essential information towards the clinical implementation of the MRT and MB radiation therapy techniques.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Animal Model ; Flash ; Glioblastoma ; Hydroxyapatite ; Mrt ; Spatially Fractionated Radiotherapy ; Virtual Histology ; X-ray Phase-contrast Imaging; Differential Phase-contrast; Computed-tomography; High-resolution; 9l Gliosarcoma; Iterative Reconstruction; Synchrotron; Image; Tissue; Beams; Microcalcifications
ISSN (print) / ISBN 2072-6694
Zeitschrift Cancers
Quellenangaben Band: 13, Heft: 19, Seiten: , Artikelnummer: 4953 Supplement: ,
Verlag MDPI
Verlagsort St Alban-anlage 66, Ch-4052 Basel, Switzerland
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Medicine (IRM)
Förderungen Deutsche Forschungsgemeinschaft