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Xu, C.S.* ; Pang, S.* ; Shtengel, G.* ; Müller, A. ; Ritter, A.T.* ; Hoffman, H.K.* ; Takemura, S.y.* ; Lu, Z.* ; Pasolli, H.A.* ; Iyer, N.* ; Chung, J.* ; Bennett, D.* ; Weigel, A.V.* ; Freeman, M.* ; van Engelenburg, S.B.* ; Walther, T.C.* ; Farese, R.V.* ; Lippincott-Schwartz, J.* ; Mellman, I.* ; Solimena, M. ; Hess, H.F.*

An open-access volume electron microscopy atlas of whole cells and tissues.

Nature 599, 147-151 (2021)
Postprint DOI PMC
Open Access Green
Understanding cellular architecture is essential for understanding biology. Electron microscopy (EM) uniquely visualizes cellular structures with nanometre resolution. However, traditional methods, such as thin-section EM or EM tomography, have limitations in that they visualize only a single slice or a relatively small volume of the cell, respectively. Focused ion beam-scanning electron microscopy (FIB-SEM) has demonstrated the ability to image small volumes of cellular samples with 4-nm isotropic voxels1. Owing to advances in the precision and stability of FIB milling, together with enhanced signal detection and faster SEM scanning, we have increased the volume that can be imaged with 4-nm voxels by two orders of magnitude. Here we present a volume EM atlas at such resolution comprising ten three-dimensional datasets for whole cells and tissues, including cancer cells, immune cells, mouse pancreatic islets and Drosophila neural tissues. These open access data (via OpenOrganelle2) represent the foundation of a field of high-resolution whole-cell volume EM and subsequent analyses, and we invite researchers to explore this atlas and pose questions.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Sprache englisch
Veröffentlichungsjahr 2021
HGF-Berichtsjahr 2021
ISSN (print) / ISBN 0028-0836
e-ISSN 1476-4687
Zeitschrift Nature
Quellenangaben Band: 599, Heft: 7883, Seiten: 147-151 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pancreatic Islet Research (IPI)
POF Topic(s) 90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502600-001
Förderungen Genentech/Roche
Carl Gustav Carus Faculty of Medicine at TU Dresden via a MeDDrive GRANT
German Center for Diabetes Research (DZD e.V.) by the German Ministry for Education and Research (BMBF)
German-Israeli Foundation for Scientific Research and Development (GIF)
German Research Foundation (DFG)
Agence nationale de la recherche (ANR)
NIAID
NIH
Howard Hughes Medical Institute (HHMI)
DOI 10.1038/s41586-021-03992-4
WOS ID WOS:000704947000001
Scopus ID 85116401084
PubMed ID 34616045
Erfassungsdatum 2021-11-18
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