PuSH - Publikationsserver des Helmholtz Zentrums München

Swaffer, M.P.* ; Kim, J.* ; Chandler-Brown, D.* ; Langhinrichs, M.* ; Marinov, G.K.* ; Greenleaf, W.J.* ; Kundaje, A.* ; Schmoller, K.M. ; Skotheim, J.M.*

Transcriptional and chromatin-based partitioning mechanisms uncouple protein scaling from cell size.

Mol. Cell 81, 4861-4875.e7 (2021)
Postprint DOI PMC
Open Access Green
Biosynthesis scales with cell size such that protein concentrations generally remain constant as cells grow. As an exception, synthesis of the cell-cycle inhibitor Whi5 "sub-scales" with cell size so that its concentration is lower in larger cells to promote cell-cycle entry. Here, we find that transcriptional control uncouples Whi5 synthesis from cell size, and we identify histones as the major class of sub-scaling transcripts besides WHI5 by screening for similar genes. Histone synthesis is thereby matched to genome content rather than cell size. Such sub-scaling proteins are challenged by asymmetric cell division because proteins are typically partitioned in proportion to newborn cell volume. To avoid this fate, Whi5 uses chromatin-binding to partition similar protein amounts to each newborn cell regardless of cell size. Disrupting both Whi5 synthesis and chromatin-based partitioning weakens G1 size control. Thus, specific transcriptional and partitioning mechanisms determine protein sub-scaling to control cell size.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cell Cycle ; Cell Size ; Cell Size Control ; Gene Expression ; Scaling; Gene-expression; Budding-yeast; Saccharomyces-cerevisiae; Protein Localization; G1/s Transcription; Cycle Control; Rna-synthesis; Copy Number; Growth; Identification
ISSN (print) / ISBN 1097-2765
e-ISSN 1097-4164
Zeitschrift Molecular Cell
Quellenangaben Band: 81, Heft: 23, Seiten: 4861-4875.e7 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Functional Epigenetics (IFE)
Förderungen Stanford MPTP T32 training grant
Human Frontier Science Program
EMBO Long-Term Postdoctoral Fellowship
Simons Foundation Fellowship of the Life Sciences Research Foundation
HHMI-Simons
NIH