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Fuenzalida Werner, J.P. ; Mishra, K. ; Stankevych, M. ; Klemm, U. ; Ntziachristos, V. ; Stiel, A.-C.

Alginate beads as a highly versatile test-sample for optoacoustic imaging.

Photoacoustics 25:100301 (2022)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Test-samples are necessary for the development of emerging imaging approaches such as optoacoustics (OA); these can be used to benchmark new labeling agents and instrumentation, or to characterize image analysis algorithms or the inversion required to form the three-dimensional reconstructions. Alginate beads (AlBes) loaded with labeled mammalian or bacterial cells provide a method of creating defined structures of controllable size and photophysical characteristics and are well-suited for both in vitro and in vivo use. Here we describe a simple and rapid method for efficient and reproducible production of AlBes with specific characteristics and show three example applications with multispectral OA tomography imaging. We show the advantage of AlBes for studying and eventually improving photo-switching OA imaging approaches. As highly defined, homogeneous, quasi point-like signal sources, AlBes might hold similar advantages for studying other agents, light-fluence models, or the impact of detection geometries on correct image formation in the near future.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Alginate ; Benchmarking ; Optoacoustic ; Photoacoustic ; Test-sample; Fluorescent Proteins; Tomography; Immobilization
Sprache englisch
Veröffentlichungsjahr 2022
Prepublished im Jahr 2021
HGF-Berichtsjahr 2021
ISSN (print) / ISBN 2213-5979
Zeitschrift Photoacoustics
Quellenangaben Band: 25, Heft: , Seiten: , Artikelnummer: 100301 Supplement: ,
Verlag Elsevier
Verlagsort Hackerbrucke 6, 80335 Munich, Germany
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
POF Topic(s) 30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-505591-004
G-505500-001
Förderungen DFG
Deutsche Forschungsgemeinschaft (DFG)
DOI 10.1016/j.pacs.2021.100301
WOS ID WOS:000742136400002
Scopus ID 85122264395
PubMed ID 35036313
Erfassungsdatum 2022-01-28
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