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Mindnich, R. ; Deluca, D. ; Adamski, J.

Identification and characterization of 17ß-hydroxysteroid dehydrogenases in the zebrafish, Danio rerio.

Mol. Cell. Endocrinol. 215, 19-30 (2004)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) are key enzymes in the final steps of steroid hormone synthesis. 17beta-HSD type 1 (HSD17B1) catalyzes the reduction of estrone to estradiol, while type 3 (HSD17B3) performs the conversion of androstenedione to testosterone. Here we present a functional genomics study of putative candidates of these enzymes in the zebrafish. By an in silico screen of zebrafish EST databases we identified three candidate homologs for both HSD17B1 and HSD 17B3. Phylogenetic analysis, unique expression patterns (RT-PCR) during etnbryogenesis and adulthood, as well as activity measurements revealed that one of the HSD17B1 candidates is the zebrafish homolog, while the other two are paralogous photoreceptor-associated retinol dehydrogenases. All three HSD17B3 candidate genes showed nearly identical, ubiquitous expressions in embryogenesis and adult tissues and were identified to be paralogs of HSD17B12 and a yet uncharacterized putative steroid dehydrogenase. Phylogenetic analysis shows that HSD17B3 and HSD17B12 are descendants from a common ancestor.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter 17ß-hydroxysteroid dehydrogenase; HSD17B1; HSD17B3; HSD17B12; Zebrafish; Embryogenesis; Evolution
ISSN (print) / ISBN 0303-7207
e-ISSN 1872-8057
Zeitschrift Molecular and Cellular Endocrinology
Quellenangaben Band: 215, Heft: 1-2, Seiten: 19-30 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Shannon
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Experimental Genetics (IEG)