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Twigger, A.-J. ; Engelbrecht, L.K. ; Bach, K.* ; Schultz-Pernice, I. ; Pensa, S.* ; Stenning, J.* ; Petricca, S. ; Scheel, C. ; Khaled, W.T.*

Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk.

Nat. Commun. 13:562 (2022)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Under normal conditions, the most significant expansion and differentiation of the adult mammary gland occurs in response to systemic reproductive hormones during pregnancy and lactation to enable milk synthesis and secretion to sustain the offspring. However, human mammary tissue remodelling that takes place during pregnancy and lactation remains poorly understood due to the challenge of acquiring samples. We report here single-cell transcriptomic analysis of 110,744 viable breast cells isolated from human milk or non-lactating breast tissue, isolated from nine and seven donors, respectively. We found that human milk largely contains epithelial cells belonging to the luminal lineage and a repertoire of immune cells. Further transcriptomic analysis of the milk cells identified two distinct secretory cell types that shared similarities with luminal progenitors, but no populations comparable to hormone-responsive cells. Taken together, our data offers a reference map and a window into the cellular dynamics that occur during human lactation and may provide further insights on the interplay between pregnancy, lactation and breast cancer.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Gene-expression; Stem-cells; Pregnancy; Tissue
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Zeitschrift Nature Communications
Quellenangaben Band: 13, Heft: 1, Seiten: , Artikelnummer: 562 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Stem Cell Research (ISF)
Förderungen CRUK Programme Foundation Award
Engineering and Physical Sciences Research Council
Science and Technology Facilities Council
ISRHML trainee bridge fund postdoctoral fellowship
Helmholtz Postdoctoral Fellowship
Cambridge Cancer Centre PhD studentship
UKRI-MRC project
UKRI-BBSRC project
Breast Cancer Now Project
Cambridge NIHR BRC Cell Phenotyping Hub