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Kleinwort, K.J.H.* ; Degroote, R.L.* ; Hirmer, S.* ; Korbonits, L.* ; Lorenz, L.* ; Scholz, A.M.* ; Hauck, S.M. ; Deeg, C.A.*

Bovine peripheral blood derived lymphocyte proteome and secretome show divergent reaction of bovine immune phenotypes after stimulation with Pokeweed mitogen.

Proteomes 10:7 (2022)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
We recently identified a deviant bovine immune phenotype characterized by hyperprolifer-ation of lymphocytes after polyclonal stimulation. This phenotype was first discovered in dams that responded to PregSure BVD vaccination by producing pathological antibodies, triggering the fatal disease “bovine neonatal pancytopenia” in calves. The aim of the study was to gain deeper insights into molecular processes occurring in lymphocytes of immune phenotypes and the effect on their secretome after immune stimulation. Two discovery proteomic experiments were performed with unstimulated and Pokeweed Mitogen (PWM) stimulated lymphocytes, using label-free LC-MS/MS. In lymphocytes, 2447 proteins were quantified, and 1204 proteins were quantified in the secretome. Quantitative proteome analysis of immune deviant and control samples after PWM stimulation revealed clear differences. The increase in abundance of IL17A, IL17F, IL8, CCL5, LRRC59, and CLIC4 was higher in controls through mitogenic stimulation. In contrast, the abundance of IFNγ, IL2, IL2RA, CD83, and CD200 increased significantly more in immune deviant lymphocytes. Additional pathway enrichment analysis of differentially secreted proteins also yielded fundamental differences between the immune phenotypes. Our study provides a comprehensive dataset, which gives novel insights into proteome changes of lymphocytes from different bovine immune phenotypes. These differences point to the development of diverse immune responses of bovine immune phenotypes after immune stimulation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cow ; Deviant Bovine Immune Phenotypes ; Differential Immune Proteomes ; Hyperproliferation ; Pokeweed Mitogen ; Polyclonal Cell Stimulation ; Quantitative Label-free Lc-ms/ms
ISSN (print) / ISBN 2227-7382
e-ISSN 2227-7382
Zeitschrift Proteomes
Quellenangaben Band: 10, Heft: 1, Seiten: , Artikelnummer: 7 Supplement: ,
Verlag MDPI
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)
Förderungen H. Wilhelm-Schaumann-Stiftung; Bayerische Tierseuchenkasse; German Ministry of Economics and Technology (via AiF); FEI