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Chan, J.T.H.* ; Kadri, S. ; Köllner, B.* ; Rebl, A.* ; Korytář, T.*

RNA-seq of single fish cells – seeking out the leukocytes mediating immunity in teleost fishes.

Front. Immunol. 13:798712 (2022)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The immune system is a complex and sophisticated biological system, spanning multiple levels of complexity, from the molecular level to that of tissue. Our current understanding of its function and complexity, of the heterogeneity of leukocytes, is a result of decades of concentrated efforts to delineate cellular markers using conventional methods of antibody screening and antigen identification. In mammalian models, this led to in-depth understanding of individual leukocyte subsets, their phenotypes, and their roles in health and disease. The field was further propelled forward by the development of single-cell (sc) RNA-seq technologies, offering an even broader and more integrated view of how cells work together to generate a particular response. Consequently, the adoption of scRNA-seq revealed the unexpected plasticity and heterogeneity of leukocyte populations and shifted several long-standing paradigms of immunology. This review article highlights the unprecedented opportunities offered by scRNA-seq technology to unveil the individual contributions of leukocyte subsets and their crosstalk in generating the overall immune responses in bony fishes. Single-cell transcriptomics allow identifying unseen relationships, and formulating novel hypotheses tailored for teleost species, without the need to rely on the limited number of fish-specific antibodies and pre-selected markers. Several recent studies on single-cell transcriptomes of fish have already identified previously unnoticed expression signatures and provided astonishing insights into the diversity of teleost leukocytes and the evolution of vertebrate immunity. Without a doubt, scRNA-seq in tandem with bioinformatics tools and state-of-the-art methods, will facilitate studying the teleost immune system by not only defining key markers, but also teaching us about lymphoid tissue organization, development/differentiation, cell-cell interactions, antigen receptor repertoires, states of health and disease, all across time and space in fishes. These advances will invite more researchers to develop the tools necessary to explore the immunology of fishes, which remain non-conventional animal models from which we have much to learn.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Korrespondenzautor
Schlagwörter Antibody ; Flow Cytometry ; Leukocytes ; Lymphocytes ; Phenotype ; Rna-seq ; Single-cell Transcriptome ; Teleost Bony Fish; Carp Cyprinus-carpio; Trout Oncorhynchus-mykiss; Toll-like Receptor; Monoclonal-antibodies; B-cells; Lymphocyte Subpopulations; Transcriptome Analysis; Adaptive Immunity; Flow-cytometry; T-lymphocytes
ISSN (print) / ISBN 1664-3224
e-ISSN 1664-3224
Zeitschrift Frontiers in Immunology
Quellenangaben Band: 13, Heft: , Seiten: , Artikelnummer: 798712 Supplement: ,
Verlag Frontiers
Verlagsort Avenue Du Tribunal Federal 34, Lausanne, Ch-1015, Switzerland
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Regenerative Biology and Medicine (IRBM)
Förderungen (MSMT- LTAUSA) - LTAUSA19108
MoMV project
state of Mecklenburg-Vorpommern
European Union
Ministry of Education, Youth and Sports-Interaction, USA of Czech Republic (MSMT-LTAUSA)