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Wagner, I.V.* ; Savchuk, I.* ; Sahlin, L.* ; Kulle, A.* ; Klöting, N. ; Dietrich, A.* ; Holterhus, P.M.* ; Dötsch, J.* ; Blüher, M.* ; Söder, O.*

De-novo and depot-specific androgen production in human adipose tissue - a source of hyperandrogenism in women with obesity.

Obes. Facts 15, 281-291 (2022)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Introduction: Obesity in women is often associated with hyperandrogenism, but the role of adipose tissue (AT) in androgen synthesis remains unclear. Therefore, we studied whether AT could be a source of androgens promoting hyperandrogenism. Methods: Subcutaneous and visceral (visc) AT was collected from lean and obese women. Androgen levels were evaluated in serum, AT, and cell-culture supernatant. Gene and protein expression of steroidogenic enzymes were determined. Results: Obese subjects had elevated serum androgen levels, which reduced after weight loss. Androgens were measurable in AT and in cell-culture supernatants of adipocytes. Steroids were higher in AT from obese women, with the highest difference for testosterone in visc AT (+7.9-fold, p = 0.032). Steroidogenic enzymes were expressed in human AT with depot-specific differences. Obese women showed a significantly higher expression of genes of the backdoor pathway and of CYP19 in visc AT. Conclusion: The whole steroidogenic machinery of the classical and backdoor pathways of steroidogenesis, and the capacity for androgen biosynthesis, were found in both AT depots and cultured adipocytes. Therefore, we hypothesize that AT is a de novo site of androgen production and the backdoor pathway of steroidogenesis might be a new pathomechanism for hyperandrogenism in women with obesity.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Adipocytes ; Androgens ; Hyperandrogenism In Women ; Obesity ; Steroidogenic Capacity
ISSN (print) / ISBN 1662-4025
e-ISSN 1662-4033
Zeitschrift Obesity Facts
Quellenangaben Band: 15, Heft: 2, Seiten: 281-291 Artikelnummer: , Supplement: ,
Verlag Karger
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Institute for Metabolism, Obesity and Vascular Research (HI-MAG)
Förderungen Universidad de las Fuerzas Armadas ESPE
Vetenskapsrådet
Institut Français de Bioinformatique
Horizon 2020 Framework Programme
European Society for Paediatric Endocrinology
Kronprinsessan Lovisas Foundation
Frimurare Barnhuset Foundation