PuSH - Publikationsserver des Helmholtz Zentrums München

Janjic, A.* ; Wange, L.E.* ; Bagnoli, J.W.* ; Geuder, J.* ; Nguyen, P.* ; Richter, D.* ; Vieth, B.* ; Vick, B. ; Jeremias, I. ; Ziegenhain, C.* ; Hellmann, I.* ; Enard, W.*

Prime-seq, efficient and powerful bulk RNA sequencing.

Genome Biol. 23:88 (2022)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost-efficient due to almost 50-fold cheaper library costs. We also validate a direct RNA isolation step, show that intronic reads are derived from RNA, and compare cost-efficiencies of available protocols. We conclude that prime-seq is currently one of the best options to set up an early barcoding bulk RNA-seq protocol from which many labs would profit.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Genomics ; Power Analysis ; Rna-seq ; Transcriptomics
ISSN (print) / ISBN 1474-760X
e-ISSN 1465-6906
Zeitschrift Genome Biology
Quellenangaben Band: 23, Heft: 1, Seiten: , Artikelnummer: 88 Supplement: ,
Verlag BioMed Central
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Apoptosis in Hematopoietic Stem Cells (AHS)
Förderungen Cyliax foundation
LMU Excellence Initiative
Deutsche Forschungsgemeinschaft