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Wenig, M. ; Bauer, K. ; Lenk, M. ; Vlot, A.C.

Analysis of innate immune responses against pathogenic bacteria in Arabidopsis, tomato, and barley.

Methods Mol. Biol. 2494, 269-289 (2022)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The immune status of plants can be evaluated by monitoring the propagation of pathogens. Plants defend themselves against pathogen attack through an intricate network of phytohormone-driven innate immune responses. Of these, salicylic acid (SA)-dependent defense responses can be assessed in planta by monitoring the propagation of biotrophic and hemi-biotrophic pathogens. Here, we describe methods to monitor the propagation of the hemi-biotrophic bacterial pathogen Pseudomonas syringae in Arabidopsis thaliana leaves. We describe protocols to (i) propagate the plants to the appropriate growth stage for infection, (ii) prepare the bacterial inoculum, (iii) inoculate plants using spray and infiltration techniques, and (iv) analyze the resulting in planta bacterial titers. The latter bacterial titers serve as a measure of plant susceptibility and negatively correlate with immunity. Based on the methods used with the A. thaliana-P. syringae model pathosystem, we include complementary methods allowing the analysis of innate immunity in the crop plants Solanum lycopersicum (tomato) in interaction with P. syringae and Hordeum vulgare (barley) in interaction with Xanthomonas translucens.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Arabidopsis Thaliana ; Bacterial Titer Determination ; Barley ; Infiltration ; Pathogen Infection ; Plant Immunity ; Pseudomonas Syringae ; Spray Inoculation ; Tomato ; Xanthomonas Translucens
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Bandtitel Environmental Responses in Plants
Zeitschrift Methods in Molecular Biology
Quellenangaben Band: 2494, Heft: , Seiten: 269-289 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biochemical Plant Pathology (BIOP)