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Grosch, J. ; Lesur, A.* ; Kler, S.* ; Bernardin, F.* ; Dittmar, G.* ; Francescato, E.* ; Hewings, S.J.* ; Jakwerth, C.A. ; Zissler, U.M. ; Heath, M.D.* ; Ollert, M.* ; Kramer, M.F.* ; Hilger, C.* ; Bilò, M.B.* ; Schmidt-Weber, C.B. ; Blank, S.

Allergen content of therapeutic preparations for allergen-specific immunotherapy of European paper wasp venom allergy.

Toxins 14:284 (2022)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Allergy to Polistes dominula (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have an impact on diagnostic and therapeutic approaches, the aim was to compare five therapeutic preparations for VIT of P. dominula venom allergy available in Spain. Products from five different suppliers were analyzed by SDS-PAGE and LC-MS/MS and compared with a reference venom sample. Three products with P. dominula venom and one product with a venom mixture of American Polistes species showed a comparable band pattern in SDS-PAGE as the reference sample and the bands of the major allergens phospholipase A1 and antigen 5 were assignable. The other product, which consists of a mixture of American Polistes species, exhibited the typical band pattern in one, but not in another sample from a second batch. All annotated P. dominula allergens were detected at comparable levels in LC-MS/MS analysis of products containing P. dominula venom. Due to a lack of genomic information on the American Polistes species, the remaining products were not analyzed by this method. The major Polistes allergens were present in comparable amounts in the majority, but not in all investigated samples of venom preparations for VIT of P. dominula venom allergy.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter American Polistes Species ; European Polistes Species ; European Paper Wasp ; Hymenoptera Venom Allergy ; Polistes Dominula ; Allergen-specific Immunotherapy ; Therapeutic Venom Extract ; Venom Immunotherapy
e-ISSN 2072-6651
Zeitschrift Toxins
Quellenangaben Band: 14, Heft: 4, Seiten: , Artikelnummer: 284 Supplement: ,
Verlag MDPI
Verlagsort Basel
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Allergy Research (IAF)
Förderungen Helmholtz Association of German Research Centres
Bencard Allergie GmbH