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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Volumetric real-time multispectral optoacoustic tomography of biomarkers.
Nat. Protoc. 6, 1121-1129 (2011)
Multispectral optoacoustic tomography (MSOT) has recently been developed to enable visualization of optical contrast and tissue biomarkers, with resolution and speed representative of ultrasound. In the implementation described here, MSOT enables operation in real-time mode by capturing single cross-sectional images in <1 ms from living small animals (e.g., mice) and other tissues of similar dimensions. At the core of the method is illumination of the object using multiple wavelengths in order to resolve spectrally distinct biomarkers over background tissue chromophores. The system allows horizontal placement of a mouse in the imaging chamber and three-dimensional scanning of the entire body without the need to immerse the mouse in water. Here we provide a detailed description of the MSOT scanner components, system calibration, selection of image reconstruction algorithms and animal handling. Overall, the entire protocol can be completed within 15-30 min for acquisition of a whole-body multispectral data set from a living mouse.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Functional photoacoustic microscopy; In-vivo; Small animals; Resolution; Algorithm; Plaques; System; Brain; Array: ; MSOT
ISSN (print) / ISBN
1754-2189
e-ISSN
1750-2799
Zeitschrift
Nature Protocols
Quellenangaben
Band: 6,
Heft: 8,
Seiten: 1121-1129
Verlag
Nature Publishing Group
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed