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Lutomska, L.M. ; Miok, V. ; Krahmer, N. ; Gonzales García, I. ; Gruber, T. ; Le Thuc, O. ; Murat, C.* ; Legutko, B. ; Sterr, M. ; Saher, G.* ; Lickert, H. ; Müller, T.D. ; Ussar, S. ; Tschöp, M.H. ; Lutter, D. ; García-Cáceres, C.

Diet triggers specific responses of hypothalamic astrocytes in time and region dependent manner.

Glia 70, 2062-2078 (2022)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Hypothalamic astrocytes are particularly affected by energy-dense food consumption. How the anatomical location of these glial cells and their spatial molecular distribution in the arcuate nucleus of the hypothalamus (ARC) determine the cellular response to a high caloric diet remains unclear. In this study, we investigated their distinctive molecular responses following exposure to a high-fat high-sugar (HFHS) diet, specifically in the ARC. Using RNA sequencing and proteomics, we showed that astrocytes have a distinct transcriptomic and proteomic profile dependent on their anatomical location, with a major proteomic reprogramming in hypothalamic astrocytes. By ARC single-cell sequencing, we observed that a HFHS diet dictates time- and cell- specific transcriptomic responses, revealing that astrocytes have the most distinct regulatory pattern compared to other cell types. Lastly, we topographically and molecularly characterized astrocytes expressing glial fibrillary acidic protein and/or aldehyde dehydrogenase 1 family member L1 in the ARC, of which the abundance was significantly increased, as well as the alteration in their spatial and molecular profiles, with a HFHS diet. Together, our results provide a detailed multi-omics view on the spatial and temporal changes of astrocytes particularly in the ARC during different time points of adaptation to a high calorie diet.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Aldehyde Dehydrogenase 1 Family Member L1 ; Arcuate Nucleus Of The Hypothalamus ; Astrocytes ; Glial Fibrillary Acidic Protein ; Hypercaloric Diet
ISSN (print) / ISBN 0894-1491
e-ISSN 1098-1136
Zeitschrift Glia
Quellenangaben Band: 70, Heft: 11, Seiten: 2062-2078 Artikelnummer: , Supplement: ,
Verlag Wiley
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Obesity (IDO)
Institute of Diabetes and Regeneration Research (IDR)
Förderungen European Union's Horizon 2020
Helmholtz Excellence Network and the Deutsche Forschungsgemeinschaft
Helmholtz Association - Initiative and Networking Fund
German Research Foundation DFG
European Research Council ERC