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Koch, F.* ; Fenouil, R.* ; Gut, M.* ; Cauchy, P.* ; Albert, T.K.* ; Zacarias-Cabeza, J.* ; Spicuglia, S.* ; de la Chapelle, A.L.* ; Heidemann, M. ; Hintermair, C. ; Eick, D. ; Gut, I.* ; Ferrier, P.* ; Andrau, J.C.*

Transcription initiation platforms and GTF recruitment at tissue-specific enhancers and promoters.

Nat. Struct. Mol. Biol. 18, 956-963 (2011)
Verlagsversion DOI PMC
Open Access Gold
Recent work has shown that RNA polymerase (Pol) II can be recruited to and transcribe distal regulatory regions. Here we analyzed transcription initiation and elongation through genome-wide localization of Pol II, general transcription factors (GTFs) and active chromatin in developing T cells. We show that Pol II and GTFs are recruited to known T cell-specific enhancers. We extend this observation to many new putative enhancers, a majority of which can be transcribed with or without polyadenylation. Importantly, we also identify genomic features called transcriptional initiation platforms (TIPs) that are characterized by large areas of Pol II and GTF recruitment at promoters, intergenic and intragenic regions. TIPs show variable widths (0.4-10 kb) and correlate with high CpG content and increased tissue specificity at promoters. Finally, we also report differential recruitment of TFIID and other GTFs at promoters and enhancers. Overall, we propose that TIPs represent important new regulatory hallmarks of the genome.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter RNA-polymerase-II; Cell-specific enhancer; Genome-wide analysis; Gene-expression; T-Cells; Regulatory elements; Locus; Activation; Chromatin; Identification
Sprache englisch
Veröffentlichungsjahr 2011
HGF-Berichtsjahr 2011
ISSN (print) / ISBN 1545-9993
e-ISSN 1545-9985
Zeitschrift Nature Structural & Molecular Biology
Quellenangaben Band: 18, Heft: 8, Seiten: 956-963 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort New York, NY
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-501490-001
PubMed ID 21765417
DOI 10.1038/nsmb.2085
Scopus ID 79961022267
Erfassungsdatum 2011-10-14
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