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Hess J. ; Unger, K. ; Maihoefer, C. ; Schuettrumpf, L. ; Weber, P. ; Marschner, S. ; Wintergerst, L. ; Pflugradt, U. ; Baumeister, P. ; Walch, A.K. ; Woischke, C.* ; Kirchner, T.* ; Werner, M.* ; Soerensen, K.* ; Baumann, M.* ; Tinhofer, I.* ; Combs, S.E. ; Debus, J.* ; Schaefer, H.* ; Krause, M.* ; Linge, A.* ; von der Gruen, J.* ; Stuschke, M.* ; Zips, D.* ; Canis, M. ; Lauber, K. ; Ganswindt, U. ; Henke, M.* ; Zitzelsberger, H. ; Belka, C.

Integration of p16/HPV DNA status with a 24-miRNA-defined molecular phenotype improves clinically relevant stratification of head and neck cancer patients.

Cancers 14:3745 (2022)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Human papillomavirus (HPV)-driven head and neck squamous cell carcinomas (HNSCC) generally have a more favourable prognosis. We hypothesized that HPV-associated HNSCC may be identified by an miRNA-signature according to their specific molecular pathogenesis, and be characterized by a unique transcriptome compared to HPV-negative HNSCC. We performed miRNA expression profiling of two p16/HPV DNA characterized HNSCC cohorts of patients treated by adjuvant radio(chemo)therapy (multicentre DKTK-ROG n = 128, single-centre LMU-KKG n = 101). A linear model predicting HPV status built in DKTK-ROG using lasso-regression was tested in LMU-KKG. LMU-KKG tumours (n = 30) were transcriptome profiled for differential gene expression and miRNA-integration. A 24-miRNA signature predicted HPV-status with 94.53% accuracy (AUC: 0.99) in DKTK-ROG, and 86.14% (AUC: 0.86) in LMU-KKG. The prognostic values of 24-miRNA- and p16/HPV DNA status were comparable. Combining p16/HPV DNA and 24-miRNA status allowed patient sub-stratification and identification of an HPV-associated patient subgroup with impaired overall survival. HPV-positive tumours showed downregulated MAPK, Estrogen, EGFR, TGFbeta, WNT signaling activity. miRNA-mRNA integration revealed HPV-specific signaling pathway regulation, including PD-L1 expression/PD-1 checkpoint pathway in cancer in HPV-associated HNSCC. Integration of clinically established p16/HPV DNA with 24-miRNA signature status improved clinically relevant risk stratification, which might be considered for future clinical decision-making with respect to treatment de-escalation in HPV-associated HNSCC.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Head And Neck Cancer ; Hnscc ; Hpv ; Mirna ; Signature ; Prediction ; Prognosis ; Mrna ; Interaction ; Signaling Pathways
ISSN (print) / ISBN 2072-6694
Zeitschrift Cancers
Quellenangaben Band: 14, Heft: 15, Seiten: , Artikelnummer: 3745 Supplement: ,
Verlag MDPI
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CCG Personalized Radiotherapy in Head and Neck Cancer (KKG-KRT)
Translational Metabolic Oncology (TMO)
Research Unit Analytical Pathology (AAP)
Institute of Radiation Medicine (IRM)