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Ozen, I.* ; Mai, H. ; de Maio, A.* ; Ruscher, K.* ; Michalettos, G.* ; Clausen, F.* ; Gottschalk, M.* ; Ansar, S.* ; Arkan, S.* ; Ertürk, A. ; Marklund, N.*

Purkinje cell vulnerability induced by diffuse traumatic brain injury is linked to disruption of long-range neuronal circuits.

Acta Neuropathol. Commun. 10:129 (2022)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Cerebellar dysfunction is commonly observed following traumatic brain injury (TBI). While direct impact to the cerebellum by TBI is rare, cerebellar pathology may be caused by indirect injury via cortico-cerebellar pathways. To address the hypothesis that degeneration of Purkinje cells (PCs), which constitute the sole output from the cerebellum, is linked to long-range axonal injury and demyelination, we used the central fluid percussion injury (cFPI) model of widespread traumatic axonal injury in mice. Compared to controls, TBI resulted in early PC loss accompanied by alterations in the size of pinceau synapses and levels of non-phosphorylated neurofilament in PCs. A combination of vDISCO tissue clearing technique and immunohistochemistry for vesicular glutamate transporter type 2 show that diffuse TBI decreased mossy and climbing fiber synapses on PCs. At 2 days post-injury, numerous axonal varicosities were found in the cerebellum supported by fractional anisotropy measurements using 9.4 T MRI. The disruption and demyelination of the cortico-cerebellar circuits was associated with poor performance of brain-injured mice in the beam-walk test. Despite a lack of direct input from the injury site to the cerebellum, these findings argue for novel long-range mechanisms causing Purkinje cell injury that likely contribute to cerebellar dysfunction after TBI.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Axonal Injury ; Central (midline) Fluid Percussion ; Cerebellum ; Demyelination ; Purkinje Cell ; Traumatic Brain Injury (tbi) ; Vdisco
e-ISSN 2051-5960
Zeitschrift Acta Neuropathologica Communications
Quellenangaben Band: 10, Heft: 1, Seiten: , Artikelnummer: 129 Supplement: ,
Verlag BioMed Central
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Tissue Engineering and Regenerative Medicine (ITERM)
Förderungen Hans-Gabriel och Alice Trolle-Wachtmeisters stiftelse för medicinsk forskning
Skånes universitetssjukhus
Vetenskapsrådet
Hjärnfonden
Crafoordska Foundation