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Riquelme-Guzmán, C.* ; Tsai, S.L.* ; Carreon Paz, K.* ; Nguyen, C.* ; Oriola, D.* ; Schuez, M.* ; Brugués, J.* ; Currie, J.D.* ; Sandoval-Guzmán, T.

Osteoclast-mediated resorption primes the skeleton for successful integration during axolotl limb regeneration.

eLife 11:e79966 (2022)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Early events during axolotl limb regeneration include an immune response and the formation of a wound epithelium. These events are linked to a clearance of damaged tissue prior to blastema formation and regeneration of the missing structures. Here, we report the resorption of calcified skeletal tissue as an active, cell-driven, and highly regulated event. This process, carried out by osteoclasts, is essential for a successful integration of the newly formed skeleton. Indeed, the extent of resorption is directly correlated with the integration efficiency, and treatment with zoledronic acid resulted in osteoclast function inhibition and failed tissue integration. Moreover, we identified the wound epithelium as a regulator of skeletal resorption, likely releasing signals involved in recruitment/differentiation of osteoclasts. Finally, we reported a correlation between resorption and blastema formation, particularly, a coordination of resorption with cartilage condensation. In sum, our results identify resorption as a major event upon amputation, playing a critical role in the overall process of skeletal regeneration.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Axolotl ; Developmental Biology ; Integration ; Osteoclasts ; Regeneration ; Regenerative Medicine ; Skeleton ; Stem Cells
ISSN (print) / ISBN 2050-084X
e-ISSN 2050-084X
Zeitschrift eLife
Quellenangaben Band: 11, Heft: , Seiten: , Artikelnummer: e79966 Supplement: ,
Verlag eLife Sciences Publications
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Pancreatic Beta Cell Research (IPI)
Förderungen Deutsche Forschungsgemeinschaft