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Forcisi, S. ; Moritz, F. ; Thompson, C.J.* ; Kanawati, B. ; Uhl, J. ; Afonso, C.* ; Bader, C.D.* ; Barsch, A.* ; Boughton, B.A.* ; Chu, R.K.* ; Ferey, J.* ; Fernandez-Lima, F.* ; Guéguen, C.* ; Heintz, D.* ; Gomez-Hernandez, M.* ; Jang, K.S.* ; Kessler, N.* ; Mangal, V.* ; Müller, R.* ; Nakabayashi, R.* ; Nicol, E.* ; Nicolardi, S.* ; Palmblad, M.* ; Paša-Tolić, L.* ; Porter, J.* ; Schmitz-Afonso, I.* ; Seo, J.B.* ; Sommella, E.* ; van der Burgt, Y.E.M.* ; Villette, C.* ; Witt, M.* ; Wittrig, A.* ; Wolff, J.J.* ; Easterling, M.L.* ; Laukien, F.H.* ; Schmitt-Kopplin, P.

Large-scale interlaboratory DI-FT-ICR MS comparability study employing various systems.

J. Am. Soc. Mass Spectrom. 33, 2203–2214 (2022)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Ultrahigh resolution mass spectrometry (UHR-MS) coupled with direct infusion (DI) electrospray ionization offers a fast solution for accurate untargeted profiling. Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers have been shown to produce a wealth of insights into complex chemical systems because they enable unambiguous molecular formula assignment even if the vast majority of signals is of unknown identity. Interlaboratory comparisons are required to apply this type of instrumentation in quality control (for food industry or pharmaceuticals), large-scale environmental studies, or clinical diagnostics. Extended comparisons employing different FT-ICR MS instruments with qualitative direct infusion analysis are scarce since the majority of detected compounds cannot be quantified. The extent to which observations can be reproduced by different laboratories remains unknown. We set up a preliminary study which encompassed a set of 17 laboratories around the globe, diverse in instrumental characteristics and applications, to analyze the same sets of extracts from commercially available standard human blood plasma and Standard Reference Material (SRM) for blood plasma (SRM1950), which were delivered at different dilutions or spiked with different concentrations of pesticides. The aim of this study was to assess the extent to which the outputs of differently tuned FT-ICR mass spectrometers, with different technical specifications, are comparable for setting the frames of a future DI-FT-ICR MS ring trial. We concluded that a cluster of five laboratories, with diverse instrumental characteristics, showed comparable and representative performance across all experiments, setting a reference to be used in a future ring trial on blood plasma.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
e-ISSN 1044-0305
Zeitschrift Journal of the American Society for Mass Spectrometry
Quellenangaben Band: 33, Heft: 12, Seiten: 2203–2214 Artikelnummer: , Supplement: ,
Verlag Elsevier
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Analytical BioGeoChemistry (BGC)
Förderungen Région Normandie
European Regional Development Fund
Labex
Horizon 2020 Framework Programme
Biological and Environmental Research
Office of Science
German Center for Diabetes Research